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Precise mapping of QTL for Hessian fly resistance in the hard winter wheat cultivar 'Overland'

文献类型： 外文期刊

作者： Xu, Yunfeng 1 ; La, Guixiao 1 ; Fatima, Nosheen 1 ; Liu, Zihui 1 ; Zhang, Lirong 1 ; Zhao, Lanfei 1 ; Chen, Ming-Shun; 1 ;

作者机构： 1.Kansas State Univ, Dept Agron, Manhattan, KS 66506 USA

2.Henan Acad Agr Sci, Ind Crop Res Inst, Zhengzhou 450002, Henan, Peoples R China

3.Hebei Acad Agr & Forestry Sci, Inst Genet & Physiol, Shijiazhuang 050051, Hebei, Peoples R China

4.Hebei Agr Univ, Dept Plant Pathol, Baoding 071001, Hebei, Peoples R China

5.USDA ARS, Hard Winter Wheat Genet Res Unit, Manhattan, KS 66506 USA

期刊名称：THEORETICAL AND APPLIED GENETICS （ 2020影响因子：5.699； 五年影响因子：5.565 ）

ISSN： 0040-5752

年卷期： 2021 年 134 卷 12 期

页码：

收录情况： SCI

摘要： Key message A major QTL for Hessian fly resistance was precisely mapped to a 2.32 Mb region on chromosome 3B of the US hard winter wheat cultivar 'Overland'. The Hessian fly (HF, Mayetiola destructor) is a destructive insect pest of wheat in the USA and worldwide. Deploying HF-resistant cultivars is the most effective and economical approach to control this insect pest. A population of 186 recombinant inbred lines (RILs) was developed from 'Overland' x 'Overley' and phenotyped for responses to HF attack using the HF biotype 'Great Plains'. A high-density genetic linkage map was constructed using 1,576 single nucleotide polymorphism (SNP) markers generated by genotyping-by-sequencing (GBS). Two quantitative trait loci (QTLs) with a significant epistatic effect on HF resistance were mapped to chromosomes 3B (QHf.hwwg-3B) and 7A (QHf.hwwg-7A) in Overland, which are located in similar chromosome regions as found for H35 and H36 in the cultivar 'SD06165', respectively. QHf.hwwg-3B showed a much larger effect on HF resistance than QHf.hwwg-7A. Five and four GBS-SNPs, respectively, in the QHf.hwwg-3B and QHf.hwwg-7A QTL intervals were converted into Kompetitive allele specific polymerase chain reaction (KASP) markers. QHf.hwwg-3B was precisely mapped to a 2.32 Mb interval (2,479,314-4,799,538 bp) using near-isogenic lines (NILs) and RILs that have recombination within the QTL interval. The US winter wheat accessions carrying contrasting alleles at KASP markers KASP-3B4525164, KASP-7A47772047 and KASP-7A65090410 showed significant difference in HF resistance. The combination of the two KASP markers KASP-3B3797431 and KASP-3B4525164 is near-diagnostic for the detection of QHf.hwwg-3B in a US winter wheat panel and can be potentially used for screening the QTL in breeding programs.

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