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Autophagy induced by Cyprinid herpesvirus 3 (CyHV-3) facilitated intracellular viral replication and extracellular viral yields in common carp brain cells

文献类型： 外文期刊

作者： Luo, Wei 1 ; Qi, Hemei 2 ; Huang, Zhihong 1 ; Guo, Min 1 ; Peng, Dikuang 1 ; Yang, Zimin 1 ; Fan, Zihan 1 ; Wang, Qing 3 ; Qin, Qiwei 1 ; Yang, Min 1 ; Lee, Xuezhu 1 ;

作者机构： 1.South China Agr Univ, Coll Marine Sci, Joint Lab Guangdong Prov & Hong Kong Reg Marine Bi, Guangzhou 510642, Peoples R China

2.Jinan Univ, Inst Biomed, Coll Life Sci & Technol, Guangzhou Jinan Biomed Res & Dev Ctr, Guangzhou 510632, Peoples R China

3.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Key Lab Aquat Anim Immune Technol, Key Lab Fishery Drug Dev,Minist Agr, Guangzhou 510380, Guangdong, Peoples R China

4.South China Agr Univ, Coll Marine Sci, 483 Wushan Rd, Guangzhou 510642, Peoples R China

关键词： CyHV-3; Autophagy; Akt/mTOR; Common carp

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.7； 五年影响因子：4.7 ）

ISSN： 1050-4648

年卷期： 2023 年 141 卷

页码：

收录情况： SCI

摘要： Autophagy is a conservative and important process that exists in all eukaryotic cells in nature. Cyprinid herpesvirus 3 (CyHV-3), also known as KHV (Koi Herpesvirus), is a pathogen that mainly infecting common carp and koi. In the present study, we identified the CcLC3B gene, with a length of 379 bp and displaying a close evolutionary relationship with other sixteen different species, the tissue distribution and expression pattern of CcLC3 were also identified. We found that CyHV-3 infection could promote autophagy in CCB cells at the early stage but inhibit autophagy at the late stage by using confocal fluorescence microscopy, transmission electron microscopy and western blotting. And we measured the protein levels associated with the Akt/mTOR signalling pathway, intracellular replication of CyHV-3 at the mRNA and protein levels as well as viral titters. Collectively, the results taken together suggested that CyHV-3 infection could promote autophagy in CCB cells at the early stage but inhibit autophagy at the late stage via mTOR and that promoting autophagy could facilitate CyHV-3 intracellular replication and extracellular viral yields in CCB cells. These findings revealed the relationship between CyHV-3 and autophagy and provided a novel treatment strategy targeting the autophagy signalling pathway against CyHV-3 infection.

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