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Genomic and functional analysis of high-level tigecycline resistant Klebsiella michiganensis co-carrying tet(X4) and tmexCD2-toprJ2 from pork

文献类型： 外文期刊

作者： Li, Yan 1 ; Xu, Lei 1 ; Li, Yuhan 1 ; Wang, Mianzhi 1 ; He, Tao 3 ; Bai, Li 4 ; Li, Ruichao 1 ; Wang, Zhiqiang 1 ;

作者机构： 1.Yangzhou Univ, Coll Vet Med, Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou, Jiangsu, Peoples R China

2.Yangzhou Univ, Inst Comparat Med, Yangzhou, Jiangsu, Peoples R China

3.Jiangsu Acad Agr Sci, Inst Food Safety & Nutr, Key Lab Control Technol & Stand Agroprod Safety &, Jiangsu Key Lab Food Qual & Safety,State Key Lab C, Nanjing, Peoples R China

4.China Natl Ctr Food Safety Risk Assessment, Natl Hlth Commiss Peoples Republ China, Key Lab Food Safety Risk Assessment, Beijing, Peoples R China

5.Yangzhou Univ, Coll Vet Med, Yangzhou, Peoples R China

关键词： Klebsiella michiganensis; tet (X4); tmexCD2-toprJ2; Real-time PCR; CRISPR; Cas9

期刊名称：INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY （ 影响因子：5.4； 五年影响因子：5.5 ）

ISSN： 0168-1605

年卷期： 2023 年 391 卷

页码：

收录情况： SCI

摘要： Tigecycline plays an important role in the clinical treatment of infections caused by multidrug-resistant pathogens. The emergence of plasmid-mediated tigecycline resistance genes tet(X) and tmexCD1-tmexJ1 has been reported in a variety of animal and animal-derived foods, and have the potential spread to humans, seriously limiting the choice of clinical medication. Herein, three ST92 Klebsiella michiganensis isolates co-harboring tet(X4) and tmexCD2-toprJ2 were collected from pork samples in Jiangsu Province, China. These K. michiganensis isolates were all multidrug-resistant isolates. Genome analysis showed that tmexCD2-toprJ2 and tet(X4) were located on IncFIB(K) and IncX1 plasmids, respectively. The IncFIB(K) plasmid pMX581-77k is a novel tmexCD2-toprJ2bearing plasmid. Worryingly, there were only a small number of SNPs between K. michiganensis isolated from pork in this study and K. michiganensis from human sources, with the possibility of clonal transmission. In addition, tet(X4) and tmexCD2-toprJ2 in K. michiganensis were able to stabilize in the absence of antibiotics. The growth curve indicated that the tmexCD2-toprJ2-positive plasmid imposed a burden on the growth of host bacteria. Interestingly, we found that the high-level resistance phenotype to tigecycline in these K. michiganensis isolates was mainly mediated by tet(X4). However, both tet(X4) and tmexCD2-toprJ2 expression were significantly elevated when host bacteria were exposed to tigecycline. This study systematically investigated K. michiganensis co-carrying tet(X4) and tmexCD2-toprJ2, emphasizing the importance for continuous surveillance of tigecyclineresistant K. michiganensis in animal-derived foods.

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