文献类型： 外文期刊
作者： Kong, Yuting 1 ; He, Mengqian 2 ; Gao, Jun 2 ; Xu, Jiehuan 2 ; Lu, Naisheng 2 ; Wu, Caifeng 2 ; Sun, Lingwei 2 ; Dai, Jianjun 2 ;
作者机构： 1.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China
2.Shanghai Acad Agr Sci, Inst Anim Husb & Vet Sci, Shanghai Municipal Key Lab Agrigenet & Breeding, Shanghai 201106, Peoples R China
3.Minist Agr & Rural Affairs, Key Lab Livestock & Poultry Resources Pig Evaluat, Shanghai 201106, Peoples R China
4.Shanghai Engn Res Ctr Breeding Pig, Shanghai 201302, Peoples R China
关键词： boar semen; cryopreservation; semen extender; energy metabolism; spermatozoa motility
期刊名称：ANIMALS （ 影响因子：2.7； 五年影响因子：3.2 ）
ISSN： 2076-2615
年卷期： 2025 年 15 卷 13 期
页码：
收录情况： SCI
摘要： The physiological functions of mammalian sperm, such as motility, hyperactivation, and capacitation, require substantial energy. This study investigates the effects of two classic cryopreservation extenders-TCG (tris-citrate-glucose) and LEY (lactose-egg yolk)-on the energy metabolism of frozen-thawed boar semen. By comparing the quality indicators, key metabolite levels, and the activities of critical enzymes involved in glycolysis and the tricarboxylic acid cycle, we aim to understand how these different semen extenders influence the spermatozoa vitality of frozen-thawed boar semen. Following thawing, the LEY-cryopreserved sperm demonstrated significantly elevated motility parameters (viability, VCL, VSL, and VAP) and enhanced plasma membrane and acrosomal integrity compared with the TCG group (p < 0.05), though both cryopreserved groups exhibited significantly reduced performance relative to fresh semen controls. Cryopreservation markedly reduced intracellular adenosine triphosphate (ATP), pyruvate, and acetyl coenzyme A (A-CoA) levels (fresh > LEY > TCG; p < 0.05). The LEY-preserved spermatozoa retained higher activities of glycolysis-related enzymes (phosphofructokinase, PFK; pyruvate kinase, PK) compared with the TCG group, which, in turn, showed elevated lactate dehydrogenase (LDH) activity. Critically, TCG-suppressed pyruvate dehydrogenase (PDH) activity (p < 0.05) coincided with diminished A-CoA, indicating impaired mitochondrial oxidative phosphorylation. These results demonstrate LEY's superior preservation of motility and membrane stability but highlight cryodamage-induced energy metabolism dysregulation, particularly TCG's disruption of the glycolysis-TCA cycle coordination essential for spermatozoa function. In conclusion, the choice of semen extender has a significant impact on the energy metabolism and overall quality of frozen-thawed semen, highlighting the importance of optimizing cryopreservation protocols for improved spermatozoa viability and functionality.
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