The molecular mechanism of gspD gene in regulating the biological characteristics, pathogenicity and virulence gene expression of Photobacterium damselae subsp. damselae
文献类型： 外文期刊
作者： Liu, Haozhe 1 ; Yu, Yongxiang 1 ; Wang, Chunyuan 1 ; Wang, Yingeng 1 ; Wu, Ronghua 4 ; Zhang, Zhiqi 1 ; Liu, Dingyuan 1 ; Liao, Meijie 1 ; Rong, Xiaojun 1 ; Li, Bin 1 ; Luo, Zhang 3 ; Zhang, Zheng 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, State Key Lab Mariculture Biobreeding & Sustainabl, Qingdao 266071, Shandong, Peoples R China
2.Laoshan Lab, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Shandong, Peoples R China
3.Tianjin Agr Univ, Coll Fisheries, Tianjin 300392, Peoples R China
4.Southwest Univ, Integrat Sci Ctr Germplasm Creat Western China CHO, Chongqing 400715, Peoples R China
5.Southwest Univ, Coll Fisheries, Aquaculture Engn Technol Res Ctr, Chongqing 400715, Peoples R China
关键词： Photobacterium damselae subsp. damselae; gspD gene; Biological characteristics; Pathogenic mechanism
期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.5； 五年影响因子：8.7 ）
ISSN： 0141-8130
年卷期： 2025 年 306 卷
页码：
收录情况： SCI
摘要： The pathogenic bacterium Photobacterium damselae subsp. damselae (PDD) has the capacity to infect various mariculture fish species. The Type II secretion system is a component of PDD, facilitating the secretion of extracellular products. The gspD gene encodes the outer membrane secretory channel protein of T2SS. To investigate the role of the gspD gene in T2SS during PDD infection, we generated a gspD gene deletion mutant of PDD (Delta gspD-PDD) using a suicide plasmid-mediated homologous recombination technique and compared the biological characteristics, virulence gene expression, and pathogenicity of Delta gspD-PDD with those of the wild-type strain (WT-PDD). Our results indicated that the hemolytic activity and phospholipase activity of Delta gspD-PDD were significantly diminished compared to WT-PDD, and the complementation strain was restored to levels similar to those of the WT-PDD. The expression levels of T2SS-related genes and the virulence genes were significantly down-regulated, while the outer membrane-related gene and flagella-related genes exhibited significant upregulation. The LD50 values of Delta gspD-PDD and its ECP in Sebastes schlegelii were 62.70-fold and 18.76-fold higher than those of WT-PDD, respectively. In summary, the mutation of the gspD gene may lead to the downregulation of T2SS-related genes, resulting in aberrant secretion of ECPs in PDD and subsequently diminishing its pathogenicity.
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