文献类型： 外文期刊

作者： Nan, Jiuhong ¹ ; Yang, Sendong ¹ ; Zhang, Xiaojian ¹ ; Leng, Tianze ¹ ; Zhuoma, Joan ¹ ; Zhuoma, Rensang ¹ ; Yuan, Jingwei ⁵ ; Pi, Jinsong ⁶ ; Sheng, Zheya ¹ ; Li, Shijun ¹ ;

作者机构： 1.Huazhong Agr Univ, Coll Anim Sci & Technol, State Key Lab Agr Anim Genet Breeding & Reprod, Minist Educ, Wuhan 430070, Peoples R China

2.Minist Nat Resources, Inst Oceanog 2, Key Lab Marine Ecosyst Dynam, Hangzhou, Peoples R China

3.Neighborhood Comm Off, Xigaze City, Peoples R China

4.Luomai Township Peoples Govt Seni Dist, Naqu City, Peoples R China

5.Chinese Acad Agr Sci, Inst Anim Sci, Beijing, Peoples R China

6.Hubei Acad Agr Sci, Inst Anim Husb & Vet, Wuhan 430064, Peoples R China

7.Huazhong Agr Univ, Key Lab Smart Farming Agr Anim, Minist Educ, Wuhan, Peoples R China

8.Huazhong Agr Univ, Hubei Hongshan Lab, Wuhan, Peoples R China

关键词： chicken; enhancer; HBZ; highland adaptation; STX2

期刊名称：ANIMAL GENETICS （ 影响因子：2.884； 五年影响因子：2.92 ）

ISSN： 0268-9146

年卷期：

页码：

收录情况： SCI

摘要： Understanding the genetic mechanism of highland adaptation is of great importance for breeding improvement of Tibetan chickens (TBC). Some studies of TBC have identified some candidate genes and pathways from multiple subgroups, but the related genetic mechanisms remain largely unknown. Different genetic backgrounds and the independent genetic basis of highland adaptation make it difficult to identity the selective region of highland adaptation with all TBC samples. In this study, we conducted pre-analysis in a large-scale population to select a TBC subgroup with the purest and highest level of highland-specific lineage for the further analysis. Finally, the 37 samples from a TBC subgroup and 19 Lahsa White chickens were used to represent the highland group for further analysis with 80 samples from five Chinese local lowland breeds as controls. Population structure analysis revealed that highland adaptation significantly affected population stratification in Chinese local chicken breeds. Genome-wide selection signal analysis identified 201 candidate genes associated with highland adaptation of TBC, and these genes were significantly enriched in calcium signaling, vascular smooth muscle contraction and the cellular response to oxidative stress pathways. Additionally, we identified a narrow 1.76 kb region containing an overlapping region between HBZ and an active enhancer, and our identified region showed a highly significant signal. The highland group selected the haplotype with high activity to improve the oxygen-carrying capacity, thus being adapted to a hypoxic environment. We also found that STX2 was significantly selected in the highland group, thus potentially reducing the oxidative stress caused by hypoxia, and that STX2 exhibited the opposite effects on highland adaptation and reproductive traits. Our findings advance our understanding of extreme environment adaptation of highland chickens, and provide some variants and genes beneficial to TBC genetic breeding improvement.