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Soybean 40S Ribosomal Protein S8 (GmRPS8) Interacts with 6K1 Protein and Contributes to Soybean Susceptibility to Soybean Mosaic Virus

文献类型： 外文期刊

作者： Hu, Ting 1 ; Guo, Dongquan 2 ; Li, Bowen 1 ; Wang, Liqun 1 ; Liu, Hui 1 ; Yin, Jinlong 1 ; Jin, Tongtong 1 ; Luan, Hexiang 3 ; Sun, Lei 1 ; Liu, Mengzhuo 1 ; Zhi, Haijian 1 ; Li, Kai 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Agr,Zhongshan Biol Breeding Lab ZSBBL,Key La, State Key Lab Crop Genet & Germplasm Enhancement &, Natl Innovat Platform Soybean Breeding & Ind Educ, Nanjing 210095, Peoples R China

2.Jilin Acad Agr Sci, Changchun 130033, Peoples R China

3.Qingdao Agr Univ, Inst Plant Genet Engn, Coll Life Sci, Qingdao 266109, Peoples R China

关键词： disease susceptibility; growth; protein interaction; virus-induced gene silencing

期刊名称：VIRUSES-BASEL （ 影响因子：4.7； 五年影响因子：4.8 ）

ISSN：

年卷期： 2023 年 15 卷 12 期

页码：

收录情况： SCI

摘要： Soybean mosaic virus (SMV), a member of Potyvirus, is the most destructive and widespread viral disease in soybean production. Our earlier studies identified a soybean 40S ribosomal protein S8 (GmRPS8) using the 6K1 protein of SMV as the bait to screen a soybean cDNA library. The present study aims to identify the interactions between GmRPS8 and SMV and characterize the role of GmRPS8 in SMV infection in soybean. Expression analysis showed higher SMV-induced GmRPS8 expression levels in a susceptible soybean cultivar when compared with a resistant cultivar, suggesting that GmRPS8 was involved in the response to SMV in soybean. Subcellular localization showed that GmRPS8 was localized in the nucleus. Moreover, the yeast two-hybrid (Y2H) experiments showed that GmRPS8 only interacted with 6K1 among the eleven proteins encoded by SMV. The interaction between GmRPS8 and 6K1 was further verified by a bimolecular fluorescence complementation (BiFC) assay, and the interaction was localized in the nucleus. Furthermore, knockdown of GmRPS8 by a virus-induced gene silencing (VIGS) system retarded the growth and development of soybeans and inhibited the accumulation of SMV in soybeans. Together, these results showed that GmRPS8 interacts with 6K1 and contributes to soybean susceptibility to SMV. Our findings provide new insights for understanding the role of GmRPS8 in the SMV infection cycle, which could help reveal potyviral replication mechanisms.

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