Rapid detection of sweepoviruses through lateral flow dipstick-based recombinase polymerase amplification
文献类型： 外文期刊
作者： Wang, He 1 ; Yang, Xiukun 1 ; Tuo, Decai 3 ; Liu, Yonghua 1 ; Zhou, Peng 3 ; Shen, Wentao 3 ; Zhu, Guopeng 1 ;
作者机构： 1.Hainan Univ, Coll Hort, Haikou 570228, Hainan, Peoples R China
2.Hainan Univ, Hainan Key Lab Qual Regulat Trop Hort Crops, Haikou 570228, Hainan, Peoples R China
3.Hainan Key Lab Protect & Utilizat Trop Bioresourc, Haikou 571101, Hainan, Peoples R China
4.Inst Trop Agr Resources, Haikou 571101, Hainan, Peoples R China
5.Chinese Acad Trop Agr Sci, Haikou 571101, Hainan, Peoples R China
6.Chinese Acad Trop Agr Sci, Key Lab Biol & Genet Resources Trop Crops, Minist Agr & Rural Affairs, Haikou 571101, Hainan, Peoples R China
7.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Haikou 571101, Hainan, Peoples R China
关键词： detection; sweepoviruses; recombinase polymerase amplification; lateral flow dipstick
期刊名称：ACTA VIROLOGICA （ 影响因子：1.827； 五年影响因子：1.632 ）
ISSN： 0001-723X
年卷期： 2022 年 66 卷 2 期
页码：
收录情况： SCI
摘要： Sweepoviruses represent a phylogenetic group of begomoviruses that cause significant sweet potato (Ipomoea batatas) production losses in various countries across the world. For rapid identification of sweepoviruses, we developed a technique based on isothermal recombinase polymerase amplification in conjunction with lateral flow dipsticks (RPA-LFD). The optimum reaction conditions for the RPA were 20 min incubation at 37 degrees C. The RPA-LFD specifically detected distinct sweepovirus species, with no other viruses infecting sweet potato causing a cross-reaction. The detection limit of the RPA-LFD was 1.0 x 10(4) copies of the target DNA molecule per reaction, and it exhibited a 10-fold greater sensitivity than the conventional PCR. Furthermore, when coupled with an alkaline polyethylene glycol-based crude genomic DNA extraction, the entire procedure was completed in 30 min without the use of any special instruments other than a water bath. Therefore, the RPA-LFD technique is a potential sweepovirus diagnostic tool that can be used in the field with fewer available resources.
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