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High-level production of cordycepin by the xylose-utilising Cordyceps militaris strain 147 in an optimised medium

文献类型： 外文期刊

作者： Long, Liangkun 1 ; Liu, Zhen 1 ; Wang, Yizhou 1 ; Lin, Qunying 3 ; Ding, Shaojun 1 ; Li, Chuanhua 4 ; Deng, Chunying 6 ;

作者机构： 1.Nanjing Forestry Univ, Coll Chem Engn, Jiangsu Coinnovat Ctr Efficient Proc & Utilisat Fo, Nanjing 210037, Peoples R China

2.Jiangsu Key Lab Chem & Utilisat Agr & Forest Bioma, Nanjing 210037, Peoples R China

3.Nanjing Inst Comprehens Utilisat Wild Plants, Nanjing 211111, Peoples R China

4.Shanghai Acad Agr Sci, Natl Engn Res Ctr Edible Fungi, Key Lab Appl Mycol Resources & Utilisat, Minist Agr, Shanghai 201403, Peoples R China

5.Shanghai Acad Agr Sci, Inst Edible Fungi, Shanghai Key Lab Agr Genet & Breeding, Shanghai 201403, Peoples R China

6.Guizhou Acad Sci, Guizhou Inst Biol, Guiyang 550009, Peoples R China

7.Guizhou Acad Sci, Guizhou Inst Biol, 40 Yanan East Rd, Guiyang 550009, Guizhou, Peoples R China

关键词： Cordyceps militaris; Cordycepin; Xylose; Medium optimisation; Transcriptome analysis

期刊名称：BIORESOURCE TECHNOLOGY （ 影响因子：11.4； 五年影响因子：10.6 ）

ISSN： 0960-8524

年卷期： 2023 年 388 卷

页码：

收录情况： SCI

摘要： Cordycepin is an important active metabolite of Cordyceps militaris. Xylose, an attractive feedstock for producing chemicals through microbial fermentation, cannot be effectively utilised by many reported C. militaris strains. Herein, a xylose-utilising C. militaris strain 147 produced the highest level of cordycepin (3.03 g/L) in xylose culture. Xylose, alanine, and ammonium citrate were determined as the main affecting factors on the cordycepin production using a Plackett-Burman design. The combination of these factors was optimised using response surface methodology, and the maximal 6.54 g/L of cordycepin was produced by the fungus in the optimal medium. Transcriptome analysis revealed that xylose utilisation upregulated the transcriptional levels of genes participating in purine and energy metabolisms in the fungus, which may facilitate the formation of precursors for cordycepin biosynthesis. This investigation provides new insights into the efficient production of cordycepin and is conducive to the valorisation of biomass rich in xylose.

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