您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Characterization and identification of enzyme-producing microflora isolated from the gut of sea cucumber Apostichopus japonicus

文献类型： 外文期刊

作者： Li Fenghui 1 ; Gao Fei 1 ; Tan Jie 1 ; Fan Chaojing 1 ; Sun Huiling 1 ; Yan Jingping 1 ; Chen Siqing 1 ; Wang Xiaojun 3 ;

作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

2.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China

3.Zhaoyuan Marine Environm Monitoring Stn, Yantai 265400, Peoples R China

关键词： Apostichopus japonicus;gut;enzyme-producing microorganisms;16S rDNA-RFLP;aquaculture;probiotics

期刊名称：CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY （ 2019影响因子：1.068； 五年影响因子：0.983 ）

ISSN： 0254-4059

年卷期： 2016 年 34 卷 1 期

页码：

收录情况： SCI

摘要： Gut microorganisms play an important role in the digestion of their host animals. The purpose of this research was to isolate and assess the enzyme-producing microbes from the Apostichopus japonicus gut. Thirty-nine strains that can produce at least one of the three digestive enzymes (protease, amylase, and cellulase) were qualitatively screened based on their extracellular enzyme-producing abilities. The enzyme-producing strains clustered into eight groups at the genetic similarity level of 100% by analyzing the restriction patterns of 16S rDNA amplified with Mbo I. Phylogenetic analysis revealed that 37 strains belonged to the genus Bacillus and two were members of the genus Virgibacillus. Enzyme-producing capability results indicate that the main enzyme-producing microflora in the A. japonicus gut was Bacillus, which can produce protease, amylase, and cellulase. Virgibacillus, however, can only produce protease. The high enzyme-producing capability of the isolates suggests that the gut microbiota play an important role in the sea cucumber digestive process.

  • 相关文献

[1]Assessment of the local environmental impact of intensive marine shellfish and seaweed farming-Application of the MOM system in the Sungo Bay, China. Zhang, Jihong,Fang, Jianguang,Wang, Wei,Jiang, Zengjie,Zhang, Jihong,Fang, Jianguang,Wang, Wei,Jiang, Zengjie,Hansen, Pia Kupka.

[2]Effects of dietary supplementation of probiotic Shewanella colwelliana WA64, Shewanella olleyana WA65 on the innate immunity and disease resistance of abalone, Haliotis discus hannai Ino. Jiang, Hai-Feng,Liu, Xiao-Lin,Wang, Gao-Xue,Jiang, Hai-Feng,Chang, Ya-Qing,Liu, Ming-Tai.

[3]Management measures to control diseases reported by tilapia (Oreochromis spp.) and whiteleg shrimp (Litopenaeus vannamei) farmers in Guangdong, China. Li, Kang,Liu, Liping,Li, Kang,Clausen, Jesper Hedegaard,Dalsgaard, Anders,Lu, Maixin.

[4]Effects of dietary administration of Shewanella haliotis D4, Bacillus cereus D7 and Aeromonas bivalvium D15, single or combined, on the growth, innate immunity and disease resistance of shrimp, Litopenaeus vannamei. Hao, Kai,Liu, Jia-Yan,Ling, Fei,Liu, Xiao-Lin,Lu, Lin,Wang, Gao-Xue,Xia, Lei. 2014

[5]Effects of probiotics dietary supplementation on growth performance; innate immunity and digestive enzymes of silver pomfret, Pampas argenteus. Gao, Quanxin,Xiao, Changfeng,Min, Minghua,Zhang, Chenjie,Peng, Shiming,Shi, Zhaohong,Xiao, Changfeng.

[6]Use of Nannochloropsis sp isolated from the East China Sea in larval rearing of sea cucumber (Apostichopus japonicus). Tan, Jie,Liu, Changlin,Li, Fenghui,Sun, Huiling,Yan, Jingping,Sun, Xiaojie,Wang, Liang,Wang, Xiaojun.

[7]A3 alpha-peptidoglycan extracted from Bifidobacterium sp could enhance immunological ability of Apostichopus japonicus. Zhang, C. Y.,Chen, G. F.,Wang, Y. Y.,Xu, Z.,Wang, Y. G.,Song, X. L..

[8]Immune responses of the sea cucumber Apostichopus japonicus to stress in two different transport systems. Tan, Jie,Sun, Xiaojie,Gao, Fei,Sun, Huiling,Yan, Jingping,Chen, Aihua,Gai, Chunlei.

[9]Substituting seaweed with corn leaf in diet of sea cucumber (Apostichopus japonicus): Effects on growth, feed conversion ratio and feed digestibility. Wu, B.,Tan, J.,Yang, A.,Xia, S.,Fu, Z.,Rahman, M. M.,Rahman, M. M.,Rajkumar, M..

[10]Effects of dietary supplementation of A3 alpha peptidoglycan on the growth, immune response and defence of sea cucumber. Zhang, C. Y.,Chen, G. F.,Wang, C. C.,Xu, Z.,Song, X. L.,Wang, Y. G.. 2014

[11]Analysis of fatty acid composition of sea cucumber Apostichopus japonicus using multivariate statistics. Xu Qinzeng,Xu Qiang,Yang Hongsheng,Gao Fei. 2014

[12]Effect of water temperature on digestive enzyme activity and gut mass in sea cucumber Apostichopus japonicus (Selenka), with special reference to aestivation. Gao Fei,Yang Hongsheng,Xu Qiang,Wang Fangyu,Liu Guangbin,Gao Fei. 2009

[13]Characterization of skin ulceration syndrome associated microRNAs in sea cucumber Apostichopus japonicus by deep sequencing. Li, Chenghua,Feng, Weida,Xia, Changge,Su, Xiurong,Jin, Chunhua,Zhou, Tingting,Zeng, Yuan,Li, Taiwu,Qiu, Lihua,Li, Taiwu. 2012

[14]Bacterial diversity of gut content in sea cucumber (Apostichopus japonicus) and its habitat surface sediment. Gao Fei,Tan Jie,Sun Huiling,Yan Jingping. 2014

[15]Development of New Microsatellite DNA Markers from Apostichopus japonicus and Their Cross-Species Application in Parastichopus parvimensis and Pathallus mollis. Liao, Meijie,Wang, Yingeng,Rong, Xiaojun,Zhang, Zheng,Li, Bin,Wang, Lan,Chen, Guiping. 2011

[16]Mechanical stress induces neuroendocrine and immune responses of sea cucumber (Apostichopus japonicus). Tan Jie,Tan Jie,Sun Huiling,Gao Fei,Yan Jingping,Wang Qingyin,Li Fenghui,Gai Chunlei,Chen Aihua. 2015

[17]Seasonal biochemical changes in composition of body wall tissues of sea cucumber Apostichopus japonicus. Gao Fei,Xu Qiang,Yang Hongsheng,Gao Fei. 2011

[18]Nitrogen and phosphorus budget of a polyculture system of sea cucumber (Apostichopus japonicus), jellyfish (Rhopilema esculenta) and shrimp (Fenneropenaeus chinensis). Li Junwei,Dong Shuanglin,Gao Qinfeng,Li Junwei,Zhu Changbo. 2014

[19]Role of animal pole protuberance and microtubules during meiosis in sea cucumber Apostichopus japonicus oocytes. Chang Yaqing,Yu Jiaping,Sun Huiling,Pang Zhenguo,Pang Zhenguo. 2010

[20]Effects of different microbes on fermenting feed for sea cucumber (Apostichopus japonicus). Jiang Yan,Mai Kangsen,Jiang Yan,Wang Yingeng,Zhang Zheng,Liao Meijie,Rong Xiaojun. 2015

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台