文献类型： 外文期刊

作者： Yao, Huang ¹ ; Yan, Lelong ¹ ; Cao, Yanyan ³ ; Ma, Zhihu ⁴ ; Zhang, Yongping ³ ; Qian, Chuntao ⁵ ;

作者机构： 1.Nanjing Agr Univ, Nanjing, Peoples R China

2.Nanjing Agr Univ, New Rural Dev Res Inst, Suzhou, Peoples R China

3.Shanghai Acad Agr Sci, Hort Res Inst, Shanghai Key Lab Protected Hort Technol, Shanghai, Peoples R China

4.Zhenjiang Inst Agr Sci Hilly Area Jiangsu Prov, Jurong, Jiangsu, Peoples R China

5.Nanjing Agr Univ, Natl Key Lab Crop Genet & Germplasm Enhancement, Nanjing, Peoples R China

关键词： Melon; Irradiated pollen; Green embryo; Chromosome doubling

期刊名称：PLANT CELL TISSUE AND ORGAN CULTURE （ 影响因子：2.4； 五年影响因子：2.6 ）

ISSN： 0167-6857

年卷期： 2024 年 159 卷 2 期

页码：

收录情况： SCI

摘要： Irradiated pollen pollination combined with embryo rescue is an effective method to induce haploid melons, but it has the disadvantage of low embryo formation efficiency. This study aims to improve the efficiency of this technique in embryo rescue. Firstly, three methods of detecting embryo-containing seeds were compared, which were light box, solid culture, and liquid culture, and the solid culture method was selected for the follow-up experiments. The results showed that a significant variation occurred in both the rate of embryo yield (ROEY) and the rate of turned green embryo (ROTGE) among 12 melon genotypes, ranging from 1.39% to 0 and from 1.05% to 0, respectively. Subsequently, melon materials G6, G61, and G6B were used as materials to design a full-factor experiment, focusing on embryo rescue time (calculated as Days After Pollination, DAP) and the rescue medium. The results showed that the highest proportions of ROEY and ROTGE were 0.89% and 0.64% at 18 DAP, while the proportions of ROEY and ROTGE were 0.71% and 0.27% at 24 DAP, and 0.7% and 0.28% at 30 DAP, respectively. Furthermore, 0.57% of ROTGE was obtained using MS medium supplemented with 0.2 mg/L 6-BA, exceeding the 0.25% obtained using the MS basal medium alone. Consequently, the method of embryo rescue at 18 DAP on MS + 0.2 mg/L 6-BA medium was referred to Treat-A and was then validated across 15 melon genotypes. The result showed a significant improvement in embryo rescue efficiency, with 1.38- and 2.65 times increase in ROEY and ROTGE respectively. Interestingly, Treat-A also resulted in a 2.05-fold increase in the Rate of regeneration plant (P1). Finally, 13 genotype haploids were treated with 200 mg/L to successfully achieve doubled haploids, with a doubling rate varying from 35% down to 0. In summary, this study presents an improved approach to embryo rescue and has acquired 151 haploids for future melon breeding. This research improves the embryo rescue efficiency of the irradiated pollen-induced haploid technique.