Physiological, transcriptomic, and metabolomic analyses of the chilling stress response in two melon (Cucumis melo L.) genotypes
文献类型： 外文期刊
作者： Diao, Qiannan 1 ; Tian, Shoubo 1 ; Cao, Yanyan 1 ; Yao, Dongwei 1 ; Fan, Hongwei 2 ; Jiang, Xuejun 3 ; Zhang, Wenxian 2 ; Zhang, Yongping 1 ;
作者机构： 1.Shanghai Acad Agr Sci, Hort Res Inst, Shanghai Key Lab Protected Hort Technol, 1018 Jinqi Rd, Shanghai 201403, Peoples R China
2.Shanghai Agr Technol Extens & Serv Ctr, Shanghai 201103, Peoples R China
3.Shanghai Jinshan Agr Technol Extens & Serv Ctr, Shanghai 201599, Peoples R China
关键词： Melon; Chilling stress; Transcriptome; Metabolome
期刊名称：BMC PLANT BIOLOGY （ 影响因子：4.8； 五年影响因子：5.4 ）
ISSN： 1471-2229
年卷期： 2024 年 24 卷 1 期
页码：
收录情况： SCI
摘要： BackgroundChilling stress is a key abiotic stress that severely restricts the growth and quality of melon (Cucumis melo L.). Few studies have investigated the mechanism of response to chilling stress in melon.ResultsWe characterized the physiological, transcriptomic, and metabolomic response of melon to chilling stress using two genotypes with different chilling sensitivity ("162" and "13-5A"). "162" showed higher osmotic regulation ability and antioxidant capacity to withstand chilling stress. Transcriptome analysis identified 4395 and 4957 differentially expressed genes (DEGs) in "162" and "13-5A" under chilling stress, respectively. Metabolome analysis identified 615 and 489 differential enriched metabolites (DEMs) were identified in "162" and "13-5A" under chilling stress condition, respectively. Integrated transcriptomic and metabolomic analysis showed enrichment of glutathione metabolism, and arginine (Arg) and proline (Pro) metabolism, with differential expression patterns in the two genotypes. Under chilling stress, glutathione metabolism-related DEGs, 6-phosphogluconate dehydrogenase (G6PDH), glutathione peroxidase (GPX), and glutathione s-transferase (GST) were upregulated in "162," and GSH conjugates (L-gamma-glutamyl-L-amino acid and L-glutamate) were accumulated. Additionally, "162" showed upregulation of DEGs encoding ornithine decarboxylase, Pro dehydrogenase, aspartate aminotransferase, pyrroline-5-carboxylate reductase, and spermidine synthase and increased Arg, ornithine, and Pro. Furthermore, the transcription factors (TFs), MYB, ERF, MADS-box, and bZIP were significantly upregulated, suggesting their crucial role in chilling tolerance of melon.ConclusionsThese findings elucidate the molecular response mechanism to chilling stress in melon and provide insights for breeding chilling-tolerant melon.
- 相关文献
作者其他论文 更多>>
-
RNA-seq-based transcriptome profiling of early fruit development in Chieh-qua and analysis of related transcription factors
作者：Du, Xuan;Liu, Na;Lu, Panling;Wang, Ying;Tian, Shoubo;Lu, Bo;Zhang, Zhaohui
关键词：Transcriptome; Early fruit development; Chieh-qua; Transcription factors; MYB_superfamily
-
Overview of research on virus-resistant breeding of melon
作者：Tian, Shoubo;Diao, Qiannan;Cao, Yanyan;Yao, Dongwei;Zhang, Hui;Du, Xuan;Zhang, Yongping;Tian, Shoubo;Diao, Qiannan;Cao, Yanyan;Yao, Dongwei;Zhang, Hui;Du, Xuan;Zhang, Yongping;Zhang, Wenxian
关键词：virus- resistance;
Cucumis melo L.; QTL; MAS; sustainable agriculture -
Improvement of embryo rescue efficiency in haploid melon (Cucumis melo L.) via irradiated pollen pollination
作者：Yao, Huang;Yan, Lelong;Yao, Huang;Yan, Lelong;Cao, Yanyan;Zhang, Yongping;Ma, Zhihu;Qian, Chuntao
关键词：Melon; Irradiated pollen; Green embryo; Chromosome doubling
-
Transcriptome Sequence Analysis of Defense Response of Resistant and Susceptible Bottle Gourd to Powdery Mildew
作者：Zhang, Zhaohui;Wang, Kuanhong;Chen, Chunhong;Yang, Xiaofeng;Tian, Shoubo;Zhu, Hongfang;Wu, Jue;Li, Jianyong;Kong, Lingjuan;Zhang, Changwei;Li, Ying;Xiao, Dong
关键词：powdery mildew resistance; bottle gourd; RNA-seq
-
Transcriptome analysis reveals association of carotenoid metabolism pathway with fruit color in melon
作者：Diao, Qiannan;Tian, Shoubo;Cao, Yanyan;Yao, Dongwei;Zhang, Yongping;Diao, Qiannan;Tian, Shoubo;Cao, Yanyan;Yao, Dongwei;Zhang, Yongping;Fan, Hongwei
关键词：
-
Comparative transcriptomic analysis of powdery mildew resistant and susceptible melon inbred lines to identify the genes involved in the response to Podosphaera xanthii infection
作者：Cao, Yanyan;Diao, Qiannan;Lu, Shijun;Zhang, Yongping;Yao, Dongwei
关键词：Melon; Powdery mildew; RNA-seq; Differentially expressed genes; Phenylpropanoid biosynthesis
-
Chloroplast genome sequence of Chongming lima bean (Phaseolus lunatus L.) and comparative analyses with other legume chloroplast genomes
作者：Tian, Shoubo;Lu, Panling;Zhang, Zhaohui;Zhang, Hui;Shen, Haibin;Jian Qiang Wu
关键词：Phaseolus lunatus; Chloroplast genome; Leguminosae; Phylogenetic relationship; Comparative analysis
