Breeding Rice lines for physio-functional food through indica 'Zhaxima' x japonica 'Nanjing 46' haploid technique
文献类型： 外文期刊
作者： Tian, Q. Q. 1 ; Li, X. 1 ; Lu, C. M. 3 ; Fang, X. W. 1 ;
作者机构： 1.China Natl Ctr Rice Improvement, Inst Food Crops, Jiangsu Acad Agr Sci, Jiangsu High Qual Rice R&D Ctr, Nanjing 210014, Peoples R China
2.Jiangsu Collaborat Innovat Ctr Modern Crop Prod, Yangzhou 225009, Jiangsu, Peoples R China
3.Nanjing Normal Univ, Coll Life Sci, Nanjing 210023, Peoples R China
关键词： Rice (Oryza sativa L); anther culture; regeneration; resistant Starch
期刊名称：FOOD PRODUCTION PROCESSING AND NUTRITION （ 影响因子：4.7； 五年影响因子：5.0 ）
ISSN：
年卷期： 2019 年 1 卷 1 期
页码：
收录情况： SCI
摘要： Resistant starch (RS) encompasses those forms of starch which are not accessible to human digestive enzymes and are fermented in the colons producing short chain fatty acids. The plant materials containing RS are few in the world. In this contribution, the culture ability of callus from anthers of F1 plants from, landraces, 'Zhaxima'(Oryza sativa var. indica, high-RS rice line with 7.705 +/- 0.142, g/100 g) x 'Nanjing 46' (Oryza sativa var. japonica, rice variety with RS content (g/100 g) of 0.200 +/- 0.001 crosses were studied for obtaining high RS rice plants. The results showed that when M8 basic induction medium was added with 1.5 mg /L 2,4-D'2 mg /LNAA and 0.3 mg /L KT, the inductivity of callus was high as 32.14% for 21 d after pretreatment at 4 degrees C for 3 d; When MS differentiation basic medium was added with 2 mg /LKT and 3 mg /L ABA, the frequency of regeneration for callus was 50.3% with only a regeneration frequency of 4.55% grown into green seedlings. The RS content in the seeds was between those of the two parents and was partially normally distributed, the highest RS contents of the regenerated plants was as high as 7.66 +/- 1.197%. This produced an efficient technology for regenerating stable rice lines with high RS and good eating quality using anthers culture.
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