Analysis of rice stripe virus whole-gene expression in rice and in the small brown planthopper by real-time quantitative PCR
文献类型： 外文期刊
作者： Li, S. 1 ; Li, X. 1 ; Sun, L. 1 ; Zhou, Y. 1 ;
作者机构： 1.Jiangsu Acad Agr Sci, Jiangsu Tech Serv Ctr Diag & Detect Plant Virus D, Inst Plant Protect, Nanjing 210014, Jiangsu, Peoples R China
2.Nanjing Agr Univ, Minist Agr, Coll Plant Protect, Dept Plant Pathol,Key Lab Monitoring & Management, Nanjing 210095, Jiangsu, Peoples R China
关键词： rice stripe virus;viral gene expression;real-time Q-PCR;rice plants;SBPH
期刊名称：ACTA VIROLOGICA （ 影响因子：1.162； 五年影响因子：1.255 ）
ISSN： 0001-723X
年卷期： 2012 年 56 卷 1 期
页码：
收录情况： SCI
摘要： Rice stripe virus (RSV) is a typical member of the Tenuivirus genus, which is transmitted mainly by the small brown planthopper (SBPH) in a persistent, circulative-propagative manner. At present, expression levels of viral genes in rice and in SBPH remain unclear. In this study, we investigated RSV gene expression in RSV-infected rice tissues and in viruliferous SBPH using a sensitive and reliable real-time quantitative PCR (Q-PCR) method. The results revealed that NS3 gene exhibited the highest abundant expression in both, rice plants and SBPH. Disease-specific protein (SP) gene was the only gene with highest expression in rice, but not in SBPH. The expression levels of other genes were lower than SP and approximately equal to each other in both, in rice and SBPH. RNA-dependent RNA polymerase (RdRp) in SBPH was present in a notably low level. Furthermore, our results also provided a basis for further investigation on how RSV coordinates its own gene expression in the interactions with its plant and insect hosts.
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