Effects of protein oxidation, cathepsins, and various freezing temperatures on the quality of superchilled sturgeon fillets
文献类型： 外文期刊
作者： Zhao, Nana 1 ; Yang, Xianqing 2 ; Li, Yujin 1 ; Wu, Haohao 1 ; Chen, Yiping 1 ; Gao, Ruichang 3 ; Xiao, Feng 4 ; Bai, Fan 5 ; Wang, Jinlin 5 ; Liu, Zunying 1 ; Gao, Xin 1 ; Zhao, Yuanhui 1 ;
作者机构： 1.Ocean Univ China, Coll Food Sci & Engn, Qingdao 266003, Peoples R China
2.Chinese Acad Fishery Sci, South China Sea Fisheries Inst, Guangzhou 510641, Peoples R China
3.Jiangsu Univ, Sch Food & Bioengn, Zhenjiang 212013, Jiangsu, Peoples R China
4.Henan Univ Sci & Technol, Coll Food & Bioengn, Luoyang 471023, Peoples R China
5.Hangzhou Qiandaohu Sturgeon Technol Co Ltd, Hangzhou 310000, Peoples R China
关键词： Sturgeon; Superchilling; Protein oxidation; Cathepsin; Microstructure
期刊名称：MARINE LIFE SCIENCE & TECHNOLOGY （ 影响因子：5.0； 五年影响因子：5.0 ）
ISSN： 2096-6490
年卷期： 2022 年 4 卷 1 期
页码：
收录情况： SCI
摘要： Many aquatic products have been stored using superchilling technology, but rarely used for the storage of sturgeon fillets. In this study, we investigated the effects of protein oxidation, cathepsin, and various freezing temperatures on the quality of superchilled sturgeon fillets. Sensory evaluation results showed that the sensory attributes of superchilled (-3 degrees C) sturgeon fillets were acceptable three times longer (18 days) than samples stored at refrigeration temperatures (4 degrees C). The sarcoplasmic protein, carbonyl, myofibrillar protein, total sulfhydryl content and the surface hydrophobicity were determined using fluorescence spectrophotometry and SDS-PAGE. Results showed that superchilling might protect myofibrillar proteins from oxidation compared to refrigeration temperatures. The activity of the three cathepsins (B, L, and H) in terms of myofibrillar, mitochondria, lysosomes, and sarcoplasm demonstrated that superchilling can inhibit cathepsins activity in sturgeon and protect its muscle structure. Microscopic observations showed that as the temperature decreased, the gap area of the muscle fibers decreased, and the deformation of cross-sectional slices was gradually reduced. In addition, the freezing rate of ice crystals produced during the freezing process influenced the muscle structure, texture, and sensory attributes. Superchilled sturgeon fillets showed good hardness, chewiness, and water retention. In conclusion, superchilling technology shows promise for its ability to extend the shelf life while maintaining the texture and sensory attributes of fresh sturgeon fillets.
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