Highly selective monoclonal antibody-based fluorescence immunochromatographic assay for the detection of fenpropathrin in vegetable and fruit samples
文献类型： 外文期刊
作者： Xu, Zi-Hong 1 ; Wang, Jun-Kai 1 ; Ye, Qiu-Xiong 2 ; Jiang, Lin-Feng 3 ; Deng, Hao 4 ; Liang, Jun-Fa 1 ; Chen, Run-Xi 1 ; Huang, Wei 1 ; Lei, Hong-Tao 1 ; Xu, Zhen-Lin 1 ; Luo, Lin 1 ;
作者机构： 1.South China Agr Univ, Guangdong Prov Key Lab Food Qual & Safety, Guangzhou 510642, Peoples R China
2.Guangzhou Inst Food Inspect, Guangzhou 510410, Peoples R China
3.Guangdong Wanlian Biotechnol Co Ltd, Guangzhou 510530, Peoples R China
4.Hainan Acad Agr Sci, Inst Agro Prod Proc & Design, Key Lab Trop Fruit & Vegetable Cold Chain Hainan P, Haikou 570100, Peoples R China
期刊名称：ANALYTICA CHIMICA ACTA （ 影响因子：6.2； 五年影响因子：5.9 ）
ISSN： 0003-2670
年卷期： 2023 年 1246 卷
页码：
收录情况： SCI
摘要： Fenpropathrin (FPT) is a typical pyrethroid pesticide that can cause chronic toxicity to humans. Herein, an anti-FPT monoclonal antibody (mAb) was elicited via a novel hapten synthesized by introducing a carboxyl-containing spacer arm in the cyclopropane moiety of FPT. Characterized by enzyme-linked immunosorbent assay (ELISA), the mAb exhibited high affinity and selectivity to FPT with a half-maximal inhibitory concentration of 31.05 mu g/L and negligible cross-reactivities with analogs of pyrethroids. Based on the mAb, a fluorescence immunochromatographic assay (FICA) for FPT detection was firstly developed. The detection limit of the FICA is 0.012 mg/kg which is much lower than the maximum residue limit of FPT for food samples. The average recoveries of FPT from spiked food samples by the FICA were 85.0-105.0%, and the obtained results were in good agreement with those of gas chromatography-tandem mass spectrometry. Overall, this work provided a reliable tool suitable for the detection of FPT residue for large-scale samples in a rapid and cost-effective manner.
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