Biosynthesis of 2-phenylethanol from styrene using engineered Escherichia coli whole cells
文献类型： 外文期刊
作者： Xiong, Tianzhen 1 ; Gao, Qiuyue 3 ; Liu, Wei 4 ; Li, Wei 1 ; Fan, Guangyan 1 ;
作者机构： 1.Xinyang Normal Univ, Coll Tea & Food Sci, Dabie Mt Lab, Xinyang 464000, Henan, Peoples R China
2.Xinyang Normal Univ, Coll Tea & Food Sci, Henan Key Lab Tea Plant Biol, Xinyang 464000, Henan, Peoples R China
3.Xinyang Univ, Coll Social Sci, 7th New Ave West, Xinyang 464000, Henan, Peoples R China
4.Jilin Acad Agr Sci, Inst Agr Qual Stand & Testing Technol, Changchun 130000, Jilin, Peoples R China
关键词： 2-phenylethanol; Escherichia coli; Cascade reaction; Whole-cell biotransformation
期刊名称：ENZYME AND MICROBIAL TECHNOLOGY （ 影响因子：3.7； 五年影响因子：3.4 ）
ISSN： 0141-0229
年卷期： 2025 年 184 卷
页码：
收录情况： SCI
摘要： 2-Phenylethanol, an aromatic alcohol with a rose scent, is widely used in the cosmetics, food, and pharmaceutical industries. We designed an efficient multi-enzyme cascade pathway for production of 2-phenylethanol from styrene as the substrate. Initially, 2-phenylethanol was produced by overexpression of styrene monooxygenase A (styA), styrene monooxygenase B (styB), styrene oxide isomerase (SOI), alcohol dehydrogenase (yahK), and glucose dehydrogenase (gdh) in Escherichia coli to give 6.28 mM 2-phenylethanol. Subsequently, plasmids with different copy numbers were employed to balance the expression of pathway enzymes to produce 10.28 mM 2phenylethanol, resulting in a 63.7 % increase in the final yield. Furthermore, the pH and temperature of the whole-cell conversion reaction were optimized, the optimum pH and temperature are 7.5 and 35 degrees C, respectively. Finally, whole-cell conversion experiment was conducted, and the production of 2-phenylethanol reached 48.17 mM within 10 h. This study provides a theoretical and practical foundation for production of 2phenylethanol.
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