文献类型： 外文期刊
作者： Zhang, Xiaoyan 1 ; Hou, Jilun 1 ; Wang, Guixing 1 ; Jiang, Hongbo 2 ; Wang, Yufen 1 ; Sun, Zhaohui 1 ; Jiang, Xiufeng; 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Beidaihe Cent Expt Stn, Qinhuangdao, Peoples R China
2.Shenyang Agr Univ, Coll Anim Sci & Vet Med, Shenyang 110161, Peoples R China
3.Chinese Acad Fishery Sci, Ctr Appl Aquat Genom, Beijing, Peoples R China
期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）
ISSN： 1932-6203
年卷期： 2015 年 10 卷 11 期
页码：
收录情况： SCI
摘要： Sterility is a serious problem that can affect all bionts. In teleosts, double haploids (DHs) induced by mitogynogenesis are often sterile. This sterility severely restricts the further application of DHs for production of clones, genetic analysis, and breeding. However, sterile DH individuals are good source materials for investigation of the molecular mechanisms of gonad development, especially for studies into the role of genes that are indispensable for fish reproduction. Here, we used the Illumina sequencing platform to analyze the transcriptome of sterile female DH Japanese flounder in order to identify major genes that cause sterility and to provide a molecular basis for an intensive study of gonadal development in teleosts. Through sequencing, assembly, and annotation, we obtained 52,474 contigs and found that 60.7% of these shared homologies with existing sequences. A total of 1225 differentially expressed unigenes were found, including 492 upregulated and 733 downregulated genes. Gene Ontology and KEGG analyses showed that genes showing significant upregulation, such as CYP11A1, CYP11B2, CYP17, CYP21, HSD3 beta, bcl2l1, and PRLR, principally correlated with sterol metabolic process, steroid biosynthetic process, and the Jak-stat signaling pathway. The significantly downregulated genes were primarily associated with immune response, antigen processing and presentation, cytokine-cytokine receptor interaction, and protein digestion and absorption. Using a co-expression network analysis, we conducted a comprehensive comparison of gene expression in the gonads of fertile and sterile female DH Japanese flounder. Identification of genes showing significantly different expression will provide further insights into DH reproductive dysfunction and oocyte maturation processes in teleosts.
- 相关文献
作者其他论文 更多>>
-
Identification candidate genes and loci associated with hypermelanosis of the blind side in Japanese flounder ( Paralichthys olivaceus) ) using the combination of RNA-Seq and BSA-Seq
作者：Liu, Mingyang;San, Lize;Zhang, Xiaoyan;Li, Bingbu;Yang, Yucong;Zheng, Xinyu;Zhang, Yitong;He, Zhongwei;Liu, Yufeng;Cao, Wei;Ren, Yuqin;Wang, Yufen;Wang, Guixing;Hou, Jilun;Liu, Mingyang;San, Lize;Zhang, Xiaoyan;Li, Bingbu;Yang, Yucong;Zheng, Xinyu;Zhang, Yitong;He, Zhongwei;Liu, Yufeng;Cao, Wei;Ren, Yuqin;Wang, Yufen;Wang, Guixing;Hou, Jilun;Liu, Mingyang;San, Lize;Zhang, Xiaoyan;Li, Bingbu;Yang, Yucong;Zheng, Xinyu;Zhang, Yitong;He, Zhongwei;Liu, Yufeng;Cao, Wei;Ren, Yuqin;Wang, Yufen;Wang, Guixing;Hou, Jilun;Zhang, Jian;Fang, Xiao
关键词：Recombinant doubled haploid; Paralichthys olivaceus; BSA-seq; Hypermelanosis; Transcriptomic analysis
-
Genetic diversity study of self-fertilized and gynogenetic Japanese flounder ( Paralichthys olivaceus) in whole genomic level
作者：San, Lize;Wang, Guixing;Zhang, Xiaoyan;Zhang, Yitong;Cao, Wei;He, Zhongwei;Liu, Yufeng;Yang, Yucong;Liu, Mingyang;Wang, Yufen;Hou, Jilun;San, Lize;Wang, Guixing;Zhang, Xiaoyan;Zhang, Yitong;Cao, Wei;He, Zhongwei;Liu, Yufeng;Yang, Yucong;Liu, Mingyang;Ren, Yuqin;Wang, Yufen;Hou, Jilun;San, Lize;Wang, Guixing;Zhang, Xiaoyan;Zhang, Yitong;Cao, Wei;He, Zhongwei;Liu, Yufeng;Yang, Yucong;Liu, Mingyang;Ren, Yuqin;Wang, Yufen;Hou, Jilun
关键词：Self-fertilization; Meiotic gynogenesis; Mitotic gynogenesis; Single nucleotide polymorphism; Genetic diversity
-
Effect of Temperature on the Early Development of Paralichthys olivaceus Otoliths
作者：Meng, Gao;Tang, Jiabao;Sun, Zhaohui;Si, Fei;Meng, Gao;Tang, Jiabao;Sun, Zhaohui;Si, Fei;Meng, Gao;Sun, Zhaohui;Si, Fei;Tang, Jiabao;Wang, Qinglin;Yu, Shanshan;Wang, Qinglin;Yu, Shanshan
关键词：
Paralichthys olivaceus ; otoliths; development; temperature -
Toxic effects of anionic polyacrylamide on the developmental stages of Oryzias melastigma embryos and larvae
作者：Zhao, Xinya;Ren, Hai;Fu, Huiru;Jin, Xiaomin;Zhao, Xinya;Zan, Jiangwei;Sun, Zhaohui;Xue, Xiangping;Si, Fei;Zhao, Xinya;Zan, Jiangwei;Sun, Zhaohui;Xue, Xiangping;Si, Fei;Zhao, Xinya;Zan, Jiangwei;Sun, Zhaohui;Xue, Xiangping;Si, Fei
关键词：Anionic polyacrylamide; Oryzias melastigma; Oxidative stress; Cardiotoxicity; Developmental toxicity
-
Community Structure and Influencing Factors of Macro-Benthos in Bottom-Seeded Marine Pastures: A Case Study of Caofeidian, China
作者：Xue, Xiangping;Sun, Zhaohui;Zan, Jiangwei;Xu, Xinjing;Liu, Xia;Gao, Song;Si, Fei;Xue, Xiangping;Sun, Zhaohui;Zan, Jiangwei;Xu, Xinjing;Liu, Xia;Gao, Song;Si, Fei;Yun, Long;Wang, Guangyu;Liu, Mingshuai
关键词：marine pastures; benthic organisms; community structure; environmental factors
-
Effect of chronic heat stress in roughskin sculpin (Trachidermus fasciatus)
作者：Liu, Zitong;Liu, Zitong;San, Lize;He, Zhongwei;Liu, Yufeng;Han, Tian;Ren, Jiangong;Ren, Yuqin;Hou, Jilun;Liu, Zitong;San, Lize;He, Zhongwei;Liu, Yufeng;Han, Tian;Ren, Jiangong;Ren, Yuqin;Hou, Jilun;Han, Tian;Gong, Chunguang
关键词：Roughskin sculpin; Heat stress; Gill tissue structure; Heat stress protein
-
Isolation, identification and the pathogenicity characterization of Pseudomonas putida 1C3 and its activation on immune responses in Japanese flounder (Paralichthys olivaceus)
作者：Zhao, Yaxian;He, Zhongwei;Liu, Yufeng;Ren, Yuqin;Ren, Jiangong;Zhang, Yitong;Wang, Yufen;Wang, Guixing;San, Lize;Hou, Jilun;Zhao, Yaxian;He, Zhongwei;Liu, Yufeng;Ren, Yuqin;Ren, Jiangong;Zhang, Yitong;Wang, Yufen;Wang, Guixing;San, Lize;Hou, Jilun;Zhao, Yaxian;He, Zhongwei;Liu, Yufeng;Ren, Yuqin;Ren, Jiangong;Zhang, Yitong;Wang, Yufen;Wang, Guixing;San, Lize;Hou, Jilun
关键词：Paralichthys olivaceus; Pseudomonas putida; Multidrug-resistant; Pathogenicity; Transcriptome
