Extraction and purification of ustiloxin A from rice false smut balls by a combination of macroporous resin and high-speed countercurrent chromatography
文献类型： 外文期刊
作者： Wang, Gang 1 ; He, Dan 1 ; Zhao, Fengchun 3 ; Hu, Junqiang 1 ; Lee, Yin-Won 4 ; Shi, Jianrong 1 ; Xu, Jianhong 1 ;
作者机构： 1.Jiangsu Acad Agr Sci, Jiangsu Key Lab Food Qual & Safety,Key Lab Agropr, State Key Lab Cultivat Base,Minist Agr & Rural Af, Minist Sci & Technol,Key Lab Control Technol & St, Nanjing, Peoples R China
2.Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang, Jiangsu, Peoples R China
3.Shandong Agr Univ, Dept Microbiol, Coll Life Sci, Key Lab Agr Microbiol, Tai An, Shandong, Peoples R China
4.Seoul Natl Univ, Sch Agr Biotechnol, Seoul 08826, South Korea
关键词： Ustiloxin A; Rice false smut; Preparative purification; Macroporous resin; High-speed counter-current chromatography; Phytotoxicity assay
期刊名称：FOOD PRODUCTION PROCESSING AND NUTRITION （ 影响因子：4.7； 五年影响因子：5.0 ）
ISSN：
年卷期： 2020 年 2 卷 1 期
页码：
收录情况： SCI
摘要： Rice false smut is an emerging plant disease worldwide. Ustiloxin A (UstA) is the major mycotoxin found in rice false smut balls, which are fungal colonies in rice florets. In this study, a new method consisting of macroporous resin column chromatography and high-speed countercurrent chromatography (HSCCC) was developed for UstA separation. UstA was extracted by a 3.81% HCOOH solution and adsorbed by XAD-4 resin. UstA was then eluted by a 40% methanol solution supplemented with 0.1% trifluoroacetic acid (TFA). Further purification was achieved by HSCCC using a two-phase solvent system consisting of n-butanol/TFA/H2O (1/0.05/1, v/v/v). Under the optimized conditions, 225 mg of UstA was obtained with a purity of 97.39% in a single run, with a final recovery of 65.2%. An inhibitory effect on seed germination of wheat and maize caused by UstA was observed in a preliminary phytotoxicity assay.
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