Cryopreservation of Noble Scallop Mimachlamys nobilis sperm: Optimization study of cooling rate and thawing temperature
文献类型： 外文期刊
作者： Li, Minghao 1 ; Fu, Zhengyi 1 ; Yu, Gang 1 ; Ma, Zhenhua 1 ;
作者机构： 1.Sanya Trop Fisheries Res Inst, Key Lab Efficient Utilizat & Proc Marine Fishery R, Sanya 572018, Peoples R China
2.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Guangzhou 510300, Peoples R China
3.Hainan Engn Res Ctr Deepsea Aquaculture & Proc, Sanya 572018, Peoples R China
4.Int Joint Res Ctr Conservat & Applicat Fishery Res, Sanya 572018, Peoples R China
5.Tianjin Agr Univ, Coll Fisheries, Tianjin 300384, Peoples R China
6.Flinders Univ Tonsley, Coll Sci & Engn, Adelaide, SA 5001, Australia
关键词： Mimachlamys nobilis; Cryopreservation; Freezing and thawing procedures; Viability; Motility rate; Ultrastructure
期刊名称：AQUACULTURE REPORTS （ 影响因子：3.7； 五年影响因子：4.0 ）
ISSN： 2352-5134
年卷期： 2024 年 39 卷
页码：
收录情况： SCI
摘要： In marine biology reproduction research, the cryopreservation of sperm is a crucial area, especially for economically valuable species. Effective freezing techniques help maintain genetic diversity and support sustainable development in aquaculture. This study examined the effects of freezing protocols and thawing methods on the cryopreservation of Mimachlamys nobilis sperm. Experimental results indicated that a cooling rate of 5 C-degrees/ min and an equilibration time of 5 minutes were the most suitable conditions for maintaining the structural and functional integrity of the sperm. Comparisons of different thawing temperatures showed that 38 C-degrees provided the best results, minimizing ice recrystallization and cellular damage. Additionally, it was found that a 10 % concentration of DMSO effectively balanced protection and cytotoxicity, maintaining sperm viability and motility. Transmission electron microscopy further revealed the impact of freezing conditions on sperm, including damage to the plasma membrane, dissolution, blurring and swelling of the acrosome and mitochondria, and cristae fragmentation and loss. This study emphasizes the importance of optimizing freezing and thawing protocols and accurately controlling cryoprotectant concentration, providing key insights for the effective long-term preservation of M.nobilis sperm. This is significant for the conservation of marine organisms and aquaculture.
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