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Identification of key genes and variants associated with boar sperm freezability using whole genome resequencing

文献类型： 外文期刊

作者： Wang, Wenjun 1 ; Bai, Yifan 1 ; Wang, Xiaokang 1 ; Zhang, Jiajun 2 ; Li, Bushe 5 ; Zhang, Hejun 5 ; Zhou, Xiang 1 ; Wang, Hongyang 3 ; Liu, Bang 1 ;

作者机构： 1.Huazhong Agr Univ, Coll Anim Sci & Technol, Key Lab Agr Anim Genet Breeding & Reprod, Minist Educ, Wuhan 430070, Peoples R China

2.Hebei Agr Univ, Coll Anim Sci & Technol, Baoding 071000, Peoples R China

3.Shanghai Acad Agr Sci, Inst Anim Husb & Vet Sci, Key Lab Livestock & Poultry Resources Pig Evaluat, Minist Agr & Rural Affairs, Shanghai 201106, Peoples R China

4.Hubei Hongshan Lab, Wuhan 430070, Peoples R China

5.Shanghai Engn Res Ctr Breeding Pig, Shanghai 201106, Peoples R China

关键词： Boar sperm freezability; Whole genome resequencing; Molecular markers

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.5； 五年影响因子：8.7 ）

ISSN： 0141-8130

年卷期： 2025 年 294 卷

页码：

收录情况： SCI

摘要： Cryopreservation induces various cryodamages to the structural or functional aspects of boar sperm, resulting in the deterioration of sperm quality. The extent of cryodamages varies significantly among different individual boars. In our study, 50 boars with either good sperm freezability (GSF) or poor sperm freezability (PSF) were selected from a population of 402 boars. These two groups exhibited significant differences in sperm quality, acrosome integrity, mitochondrial membrane potential, and plasma membrane integrity. Subsequent wholegenome resequencing and FST analysis of GSF and PSF boars uncovered genetic differentiation among 5632 genes. Spermatogenesis, sperm structure, and lipid composition were identified as the potential factors, and 35 genes, such as RNF8, PACRG, CADM1, SPAG16, PPP3CA and MFF, were identified as key candidate genes associated with sperm freezability. Furthermore, by using MassARRAY genotyping and a general linear model, nine variants, including seven single nucleotide polymorphisms (SNPs) and two insertion variants, were found to be significantly correlated with sperm freezability. These variants, located within the candidate genes, represent potential molecular markers for sperm freezability. Our research contributes novel insights into the genetic mechanisms underlying freezability differences in boar sperm and identifies candidate genes and key molecular markers that could be applied in boar breeding practices.

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