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The irregular developmental duration mainly caused by the broad-complex in Chilo suppressalis

文献类型： 外文期刊

作者： Zhang, Zhi-Ling 1 ; Xu, Qing-Yu 2 ; Zhang, Ru 2 ; Shen, Chen 1 ; Bao, Hai-Bo 2 ; Luo, Guang-Hua 1 ; Fang, Ji-Chao 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Plant Protect, Nanjing 210095, Peoples R China

2.Jiangsu Acad Agr Sci, Inst Plant Protect, Jiangsu Key Lab Food, Safety State Key Lab Cultivat Base Minist Sci & Te, Nanjing 210014, Peoples R China

关键词： Broad-complex isoforms; Chilo suppressalis; Developmental duration; RNA interference; CRISPR/Cas9

期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：4.0； 五年影响因子：4.5 ）

ISSN： 0048-3575

年卷期： 2024 年 204 卷

页码：

收录情况： SCI

摘要： Chilo suppressalis, a critical rice stem borer pest, poses significant challenges to rice production due to its overlapping generations and irregular developmental duration. These characteristics complicate pest management strategies. According to the dynamic analysis of the overwintering adults of C. suppressalis in fields, it indicates that the phenomenon of irregular development of C. suppressalis exists widely and continuously. This study delves into the potential role of the Broad-Complex (Br-C) gene in the developmental duration of C. suppressalis. Four isoforms of Br-C, named CsBr-C Z1, CsBr-C Z2, CsBr-C Z4, and CsBr-C Z7, were identified. After CsBr-Cs RNAi, the duration of larva development spans extended obviously. And, the average developmental duration of dsCsBr-Cs feeding individuals increased obviously. Meanwhile, the average developmental duration of the dsCsBr-C Z2 feeding group was the longest among all the RNAi groups. After dsCsBr-Cs feeding continuously, individuals pupated at different instars changed obviously: the proportion of individuals pupated at the 5th instar decreased and pupated at the 7th instar or higher increased significantly. Moreover, the pupation rate of dsCsBr-Cs (except dsCsBr-C Z7) were significantly lower than that of dsGFP. The same results were obtained from the mutagenesis in CsBr-C genes mediated by CRISPR/Cas9. The average developmental duration of CsBr-Cs knockout individuals was significantly prolonged. And, the instar of pupation in knockout individuals was also delayed significantly. In conclusion, this work showed that CsBr-Cs played a crucial role in pupal commitment and affected the developmental duration of C. suppressalis significantly.

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