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Bacteriophage-based control of Salmonella on table eggs and breeding eggs in poultry

文献类型： 外文期刊

作者： Wang, Yanyan 1 ; Li, Huimin 2 ; Buttimer, Colin 4 ; Zhang, Hui 2 ; Zhou, Yan 2 ; Ji, Linchun 2 ; Li, Yue 2 ; Wang, Ran 2 ; Bao, Hongduo 2 ;

作者机构： 1.Univ Jiangsu, Sch Food & Bioengn, Zhenjiang 212013, Peoples R China

2.Jiangsu Acad Agr Sci, Inst Food Safety & Nutr, Jiangsu Key Lab Food Qual & Safety, State Key Lab Cultivat Base MOST, Nanjing 210014, Peoples R China

3.Nanjing Agr Univ, Coll Vet Med, Nanjing 210095, Peoples R China

4.Univ Coll Cork, APC Microbiome Inst, Cork T12 YT20, Ireland

5.Huaiyin Inst Technol, Coll Life Sci & Food Engn, Huaian 223003, Peoples R China

关键词： Salmonella phage; biological characteristics; genome sequencing; biocontrol; breeding eggs

期刊名称：POULTRY SCIENCE （ 影响因子：4.2； 五年影响因子：4.5 ）

ISSN： 0032-5791

年卷期： 2025 年 104 卷 4 期

页码：

收录情况： SCI

摘要： Salmonella is a major foodborne pathogen, that poses a serious threat to poultry farm production. Phage-based biocontrol offered a promising alternative strategy to eradicate the persistent and challenging infections caused by Salmonella in this setting. This study isolated and purified the lytic Salmonella phage vB_SenM_BP13076 (simple as BP13076) using its host strain Salmonella Enteritidis ATCC 13076. Its genome was extensively analyzed, and its potential biocontrol application towards eggs was investigated. Morphological analyses revealed that phage BP13076 is characterized by an icosahedral head and a contractile tail, placing it among Caudoviricetes. The phage demonstrated a broad host range, lysing 66 out of 68 tested Salmonella strains, including eight globally prevalent serovars. Moreover, it also exhibited a short latent period of approximately 5 min and a burst size of about 105 PFU/cell. It also demonstrates good thermal stability and a wide pH range tolerance. The genome of phage BP13076 consists of 160, 318 bp of dsDNA with a G + C content of 37.13% with nucleotide homology placing it among phages of the genus Gelderlandvirus. Notably, the genomic analysis revealed no known genes associated with virulence, antibiotic resistance, or lysogeny, making it a safe candidate for biocontrol applications. In vitro, bacteriostatic tests indicated higher MOI (multiplicity of infection), resulting in a more significant reduction in Salmonella counts. When applied to table and breeding eggs, phage BP13076 at MOIs of 100 and 1000 resulted in a significant decrease in Salmonella levels compared to the positive control groups. These findings highlight the efficacy of phage BP13076 as a promising biocontrol agent for managing Salmonella contamination and transmission for table and breeding eggs, offering a foundation for its potential application in the prevention and control of Salmonella in the poultry industry.

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