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Heat-shock protein 70 reduces apoptosis in the gills and hepatopancreas of Marsupenaeus japonicus under low-temperature stress

文献类型： 外文期刊

作者： Ren, Xianyun 1 ; Bian, Xueqiong 1 ; Jia, Shaoting 1 ; Gao, Tian 1 ; Wang, Junxia 5 ; Wang, Jiajia 1 ; Liu, Ping 1 ; Li, Jian 1 ; Li, Jitao 1 ;

作者机构： 1.Chinese Acad Fishery Sci, State Key Lab Mariculture Biobreeding & Sustainabl, Yellow Sea Fisheries Res Inst, Qingdao 266071, Shandong, Peoples R China

2.Qingdao Marine Sci & Technol Ctr, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Shandong, Peoples R China

3.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China

4.Dalian Ocean Univ, Coll Fisheries & Life Sci, Dalian 116023, Peoples R China

5.Rizhao Ocean & Fishery Res Inst, Marine Sci Res Ctr, Rizhao 276800, Shandong, Peoples R China

关键词： Marsupenaeus japonicus; MjHSP70; MjHSF1; Low temperature stress; Apoptosis

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.5； 五年影响因子：8.7 ）

ISSN： 0141-8130

年卷期： 2025 年 307 卷

页码：

收录情况： SCI

摘要： The heat shock response (HSR) has important functions in a cell's reaction to stresses. The HSR is regulated and effected by heat shock proteins (HSPs) and heat shock factors (HSFs). Herein, the full-length cDNA of Marsupenaeus japonicus heat shock protein 70 (MjHSP70) was isolated and sequenced. The MjHSP70 cDNA comprised 2346 bp with an open reading frame of 1959 bp, encoding a protein of 653 amino acids. Both MjHSF1 and MjHSP70 are widely expressed across tissues. In response to low temperature stress, MjHSF1 and MjHSP70 expression levels were significantly upregulated in the gill and hepatopancreas. After RNA interference-mediated knockdown of MjHSP70, the mortality rate of M. japonicus increased significantly under low temperature stress, apoptotic genes, e.g., Mjcaspase3 and Mjbcl2, showed significantly upregulated expression, and the apoptotic cell count also increased. Additionally, dual luciferase assays showed that MjHSF1 activated MjHSP70 transcription, and MjHSP70 expression was downregulated after MjHSF1 silencing. The results of glutathione-S-transferase GST pull-down assays indicated that MjHSP70 interacted with MjHSF1's DNA binding domain. Our findings suggested that the MjHSF1-MjHSP70 axis exerts a vital function in M. japonicus' immune response to cold stress.

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