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Dual-responsive zeolitic imidazolate framework-90 for the combined detection and intracellular imaging of ATP and ROS

文献类型： 外文期刊

作者： Shi, Xinran 1 ; Ji, Liang 1 ; Hu, Yuanyuan 1 ; Gu, Jinyu 1 ; Wang, Liming 1 ; Lu, Wenwen 1 ; Meng, Jiali 1 ; Du, Yan 1 ; Huang, Lingzhong 3 ; Nie, Dongxia 2 ; Yu, Yanyan 1 ;

作者机构： 1.Xuzhou Med Univ, Jiangsu Key Lab New Drug Res & Clin Pharm, Xuzhou 221004, Jiangsu, Peoples R China

2.Shanghai Acad Agr Sci, Inst Agrofood Stand & Testing Technol, 1000 Jinqi Rd, Shanghai 201403, Peoples R China

3.Shenzhen Power Supply Bur Co Ltd, Shenzhen 518020, Peoples R China

关键词： Zeolitic imidazolate framework-90; Reactive oxygen species; ATP; Intracellular imaging; Fluorescence analysis; Alzheimer's disease

期刊名称：SENSORS AND ACTUATORS B-CHEMICAL （ 影响因子：9.221； 五年影响因子：7.676 ）

ISSN：

年卷期： 2022 年 363 卷

页码：

收录情况： SCI

摘要： Since adenosine triphosphate (ATP) and reactive oxygen species (ROS) are closely related to neuron functions and the pathogenesis of Alzheimer's disease, herein, the first example of using a dual-responsive zeolitic imidazolate framework-90 (ZIF-90) to fabricate a single fluorescent probe, CuNCs/ZIF@P1-P2/ZIF, was reported, which specifically and sensitively responded to intracellular level fluctuations of ATP and ROS both in neuronal and tumor cells without obvious cross-talking. Through a stepwise in situ growth method, ROS-responsive glutathione-stabilized copper nanoclusters (GSH-CuNCs) and double tagged ATP aptamer hybrid chain (P1 P2) were prepared and respectively encapsulated into the inner and outer ZIF-90. The obtained CuNCs/ZIF@P1P2/ZIF probe as a tandem system could firstly respond to ATP in an "off-to-on" mode, in which, the quenched fluorescence of FAM by BHQ1 attached on P2 was recovered through the competitive displacement between ATP and ZIF-90, leading to the release of FAM from ZIF-90. Upon collapse of the outer ZIF-90, the strong emission of CuNCs enclosed in the inner ZIF-90 was subsequently quenched in the presence of ROS. Accompanied with high sensitivity, selectivity and accuracy, the resultant fluorescent probe exhibited good performance and properties for tracking the level variations of ATP and ROS in SH-SY5Y and Hela cells under different stimulations. This strategy highlighted the potential of the ZIF-90 based nanocomposite for multi-targets detection, and also provided a value tool for the early diagnosis of diseases.

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