Genetic signatures associated with the virulence of porcine epidemic diarrhea virus AH2012/12
文献类型: 外文期刊
作者: Peng, Qi 1 ; Fan, Baochao 1 ; Song, Xu 1 ; He, Wenlong 1 ; Wang, Chuanhong 1 ; Zhao, Yongxiang 1 ; Guo, Weilu 1 ; Zhang, Xue 1 ; Liu, Shiyu 1 ; Gao, Jie 1 ; Li, Kemang 1 ; Zhang, Baotai 1 ; Zhou, Jinzhu 1 ; Li, Yunchuan 1 ; Guo, Rongli 1 ; Li, Bin 1 ;
作者机构: 1.Jiangsu Acad Agr Sci, Inst Vet Med, Jiangsu Key Lab Food Qual & Safety, State Key Lab Cultivat Base,Minist Sci & Technol, Nanjing 210014, Peoples R China
2.Jiangxi Agr Univ, Coll Biosci & Engn, Nanchang, Jiangxi, Peoples R China
3.Yangzhou Univ, Jiangsu Coinnovat Ctr Prevent & Control, Jiangsu Key Lab Zoonosis, Important Anim Infect Dis & Zoonoses, Yangzhou, Jiangsu, Peoples R China
4.Guotai Taizhou Ctr Technol Innovat Vet Biol, Taizhou, Jiangsu, Peoples R China
5.Hebei Agr Univ, Coll Vet Med, Baoding, Hebei, Peoples R China
6.Jiangsu Univ, Acad Life Sci, Zhenjiang, Jiangsu, Peoples R China
7.Nanjing Tech Univ, Sch Pharmaceut Sci, Nanjing, Jiangsu, Peoples R China
8.Nanjing Agr Univ, Coll Vet Med, Nanjing, Jiangsu, Peoples R China
9.Guizhou Univ, Coll Anim Sci, Guiyang, Guizhou, Peoples R China
关键词: PEDV; reverse genetics; S gene; pathogenicity; fusogenicity
期刊名称:JOURNAL OF VIROLOGY ( 影响因子:5.4; 五年影响因子:4.9 )
ISSN: 0022-538X
年卷期: 2023 年
页码:
收录情况: SCI
摘要: Porcine epidemic diarrhea virus (PEDV) is a highly pathogenic swine coronavirus causing severe diarrhea and high mortality to piglets. PEDV strain AH2012/12 isolated from a diarrheal piglet has been passaged in vitro for over 102 passages. Viral infection assay revealed that PEDV AH2012/12-P102 (the 102nd passage of AH2012/12) showed an enhanced fusogenicity than the wild-type AH2012/12. Animal experiments demonstrated that AH2012/12-P102 is an attenuated PEDV strain as shown by the evidence of no mortality, extremely low virus shedding, and no sign of diarrhea in the AH2012/12-P102 challenged piglets. Compared with AH2012/12, AH2012/12-P102 had two obvious deletions in the genome, one deletion is in the S1 gene and the second deletion contains the carboxy-terminus of the S2 gene and the start codon of ORF3. Using the reverse genetic system of PEDV, we generated a series of recombinant PEDVs with deletions based on the deletion in the genome of AH2012/12-P102. Viral infection assays indicated that the second deletion could enhance the fusogenicity of PEDV AH2012/12. Animal experiments showed that the first deletion could reduce the virulence but not fully attenuate AH2012/12, but the second deletion could attenuate PEDV AH2012/12 in vivo. Further animal experiments indicated that the recombinant PEDV with deleted carboxy-terminus of S gene induced higher IgG, IgA, neutralization antibodies, and protection effects against virus challenge than the killed vaccine. Collectively, our data demonstrated two genetic features associated with the virulence of PEDV AH2012/12 and provided a promising method for the development of attenuated vaccine candidates for PEDV.
- 相关文献
作者其他论文 更多>>
-
The glycosylation sites in RBD of spike protein attenuate the immunogenicity of PEDV AH2012/12
作者:Zhang, Gege;Wang, Jiaxiang;Zhang, Gege;Peng, Qi;Liu, Shiyu;Fan, Baochao;Wang, Chuanhong;Song, Xu;Cao, Qiuxia;Li, Chengcheng;Xu, Hong;Lu, Hongting;Bao, Meiying;Yang, Shanshan;Li, Yunchuan;Li, Bin;Liu, Shiyu;Li, Bin;Liu, Shiyu;Fan, Baochao;Yang, Shanshan;Li, Yunchuan;Li, Bin;Fan, Baochao;Yang, Shanshan;Li, Yunchuan;Li, Bin
关键词:PEDV; RBD; Glycosylation; Reverse genetics; Pathogenicity; Immunogenicity
-
Nonstructural protein 14 of PDCoV promotes complement C3 expression via the activation of p38-MAPK-C/EBP pathway
作者:Chen, Zhuoqi;Fan, Liyuan;Shang, Hongqi;Xiao, Li;Wang, Wei;Guo, Rongli;Li, Jizong;Chen, Zhuoqi;Fan, Liyuan;Shang, Hongqi;Wang, Wei;Guo, Rongli;Li, Jizong;Li, Jizong;Li, Jizong;Li, Jizong;Li, Jizong;Zhong, Chunyan
关键词:PDCoV; C3; Nsp14; Complement; C/EBP-beta
-
Inhibition of Hyperglycemia and Hyperlipidemia by Blocking Toll-like Receptor 4: Comparison of Wild-Type and Toll-like Receptor 4 Gene Knockout Mice on Obesity and Diabetes Modeling
作者:Zhao, Xingyu;Huang, Wuyang;Zhao, Xingyu;Zheng, Jiawei;Huang, Wuyang;Wang, Jing;Huang, Wuyang;Li, Bin
关键词:TLR4; glycolipid metabolism; obesity and diabetes modeling; knockout
-
Homoeologous exchanges contribute to branch angle variations in rapeseed: Insights from transcriptome, QTL-seq and gene functional analysis
作者:Sun, Chengming;Zhou, Xiaoying;Fu, Sanxiong;Wang, Xiaodong;Peng, Qi;Gao, Jianqin;Chen, Feng;Zhang, Wei;Hu, Maolong;Zhang, Jiefu;Wu, Jian;Liu, Huimin;Wang, Youping;Xue, Zhifei;Fu, Tingdong;Yi, Bin
关键词:Rapeseed; Branch angle; Homoeologous exchange; WGCNA; QTL-seq; WRKY40
-
Lipidomics reveals the significance and mechanism of the cellular ceramide metabolism for rotavirus replication
作者:Tao, Ran;Cheng, Xi;Gu, Laqiang;Zhou, Jinzhu;Zhu, Xuejiao;Zhang, Xuehan;Guo, Rongli;Wang, Wei;Li, Bin;Tao, Ran;Cheng, Xi;Gu, Laqiang;Zhou, Jinzhu;Zhu, Xuejiao;Zhang, Xuehan;Guo, Rongli;Wang, Wei;Li, Bin;Tao, Ran;Cheng, Xi;Gu, Laqiang;Zhou, Jinzhu;Zhu, Xuejiao;Zhang, Xuehan;Guo, Rongli;Wang, Wei;Li, Bin;Cheng, Xi;Li, Bin;Gu, Laqiang;Li, Bin
关键词:rotavirus; lipidomics; ceramide; inhibition; viral replication; apoptosis
-
First Specific Detection of Mammalian Orthoreovirus from Goats Using TaqMan Real-Time RT-PCR Technology
作者:Mao, Li;Li, Xia;Cai, Xuhang;Li, Wenliang;Li, Jizong;Yang, Shanshan;Li, Bin;Mao, Li;Li, Xia;Cai, Xuhang;Li, Wenliang;Li, Jizong;Yang, Shanshan;Li, Bin;Mao, Li;Li, Xia;Cai, Xuhang;Li, Wenliang;Li, Jizong;Yang, Shanshan;Li, Bin;Mao, Li;Li, Xia;Cai, Xuhang;Li, Wenliang;Li, Jizong;Yang, Shanshan;Li, Bin;Mao, Li;Li, Xia;Li, Wenliang;Li, Jizong;Suolang, Sizhu;Li, Bin;Mao, Li;Li, Wenliang;Li, Jizong;Yang, Shanshan;Li, Bin;Cai, Xuhang;Zhai, Junjun
关键词:mammalian orthoreovirus; TaqMan qRT-PCR; detection; goat; sheep
-
A Novel Polymer Nanoparticle Polydimethyl Diallyl Ammonium Chloride as An Adjuvant Enhances the Immune Response of SARS-CoV-2 Subunit Vaccine
作者:Ren, Lili;Ouyang, Chengcheng;Zhao, Shuqing;Zheng, Qiqi;Guo, Weilu;Zhang, Wei;Zhao, Shuqing;Zheng, Qiqi;Guo, Weilu;Fan, Baochao;Zhou, Jinzhu;Hu, Mi;Li, Jizong;Li, Bin;Fan, Baochao;Li, Jizong;Li, Bin;Fan, Baochao;Li, Jizong;Li, Bin;Fan, Baochao;Li, Jizong;Li, Bin
关键词:adjuvant; polydimethyl diallyl ammonium chloride; SARS-CoV-2; subunit vaccine