文献类型： 外文期刊

作者： Wang, Xiao-jing ¹ ; Peng, Xu-qian ² ; Shu, Xiao-chun ³ ; Li, Yu-hang ³ ; Wang, Zhong ³ ; Zhuang, Wei-bing ³ ;

作者机构： 1.Guizhou Univ, Key Lab Plant Resource Conservat & Germplasm Inno, Minist Educ, Guiyang 550025, Guizhou, Peoples R China

2.Guizhou Acad Agr Sci, Inst Hort, Guiyang 550006, Guizhou, Peoples R China

3.Jiangsu Prov & Chinese Acad Sci, Inst Bot, Jiangsu Prov Platform Conservat & Utilizat Agr Ge, Nanjing Bot Garden Mem Sun Yat Sen, Nanjing 210014, Peoples R China

关键词： Colored-leaf poplar; Anthocyanin biosynthesis; PdeHLH genes; Phylogenetic analysis; Structure analysis; Expression pattern

期刊名称：BMC GENOMICS （ 影响因子：4.547； 五年影响因子：4.931 ）

ISSN： 1471-2164

年卷期： 2022 年 23 卷 1 期

页码：

收录情况： SCI

摘要： Basic helix-loop-helix (bHLH) proteins are transcription factors (TFs) that have been shown to regulate anthocyanin biosynthesis in many plant species. However, the bHLH gene family in Populus deltoids has not yet been reported. In this study, 185 PdbHLH genes were identified in the Populus deltoids genome and were classified into 15 groups based on their sequence similarity and phylogenetic relationships. Analysis of the gene structure, chromosome location and conserved motif of the PdbHLH genes were performed by bioinformatic methods. Gene duplication analyses revealed that 114 PdbHLH were expanded and retained after WGD/segmental and proximal duplication. Investigation of cis-regulatory elements of PdbHLH genes indicated that many PdbHLH genes are involved in the regulation of anthocyanin biosynthesis. The expression patterns of PdbHLHs were obtained from previous data in two colored-leaf poplar (QHP and JHP) and green leaf poplar (L2025). Further analysis revealed that 12 candidate genes, including 3 genes (PdbHLH57, PdbHLH143, and PdbHLH173) from the subgroup III(f) and 9 gene from other groups, were positively associated with anthocyanin biosynthesis. In addition, 4 genes (PdbHLH4, PdbHLH1, PdbHLH18, and PdbHLH164) may be involved in negatively regulating the anthocyanin biosynthesis. These results provide a basis for the functional characterization of bHLH genes and investigations on the molecular mechanisms of anthocyanin biosynthesis in colored-leaf poplar.