您好，欢迎访问黑龙江省农业科学院 机构知识库！
黑龙江省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Efficacy Assessment of Phage Therapy in Treating Staphylococcus aureus-Induced Mastitis in Mice

文献类型： 外文期刊

作者： Teng, Fei 1 ; Xiong, Xiaoyu 1 ; Zhang, Songsong 1 ; Li, Guiwei 2 ; Wang, Ruichong 3 ; Zhang, Lanlan 4 ; Wang, Xiaona 1 ; Zhou, Han 1 ; Li, Jiaxuan 1 ; Li, Yijing 1 ; Jiang, Yanping 1 ; Cui, Wen 1 ; Tang, Lijie 1 ; Wang, Li 1 ; Qiao, Xinyuan 1 ;

作者机构： 1.Northeast Agr Univ, Heilongjiang Key Lab Anim Dis Control & Pharmaceu, Dept Prevent Vet Med, Coll Vet Med, Harbin 150038, Peoples R China

2.Heilongjiang Acad Agr Sci, Branch Anim Husb & Vet, Qiqihar 161000, Peoples R China

3.Heilongjiang Prov Ctr Dis Control & Prevent, Dept Radiol Protect, Harbin 150030, Peoples R China

4.Heilongjiang Fishery Technol Extens Stn, Promot Demonstrat Dept, Harbin 150030, Peoples R China

关键词： Staphylococcus aureus; mastitis; drug-resistant; phage

期刊名称：VIRUSES-BASEL （ 影响因子：5.818； 五年影响因子：5.811 ）

ISSN：

年卷期： 2022 年 14 卷 3 期

页码：

收录情况： SCI

摘要： The primary aim of this study was to evaluate the efficacy of phage against mastitis induced by drug-resistant S. aureus in a mouse model. In this study, five S. aureus phages-4086-1, 4086-2, 4086-3, 4086-4, and 4086-6-were isolated from milk samples secreted by mastitis cows. Transmission electron microscopy showed that all the five phages had icosahedral heads and short non-contractile tails, which are typical characteristics of the family Podoviridae. All these phages were species-specific against S. aureus. The one-step growth curve showed a short latency period (10-20 min) and high burst size (up to 400 PFU/infected cell). To evaluate the effectiveness of the phage 4086-1 in the treatment against mastitis, a mouse model of mastitis was challenged with drug-resistant S. aureus. The results showed the proliferation of S. aureus in the mammary glands was significantly inhibited after treating by phage 4086-1. The concentrations of TNF-alpha and IL-6 decreased significantly, which demonstrated the phages could effectively alleviate the inflammatory responses. Furthermore, the histopathological analysis showed that inflammatory infiltration in the mammary glands was significantly reduced. These results demonstrate that phage may be a promising alternative therapy against mastitis caused by drug-resistant S. aureus.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：黑龙江省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台