Arabinose Plays an Important Role in Regulating the Growth and Sporulation of Bacillus subtilis NCD-2
文献类型： 外文期刊
作者： Fu, Yifan 1 ; Liu, Xiaomeng 2 ; Su, Zhenhe 2 ; Wang, Peipei 2 ; Guo, Qinggang 2 ; Ma, Ping 2 ;
作者机构： 1.Agr Univ Hebei, Coll Plant Protect, Baoding 071000, Peoples R China
2.Hebei Acad Agr & Forestry Sci, Inst Plant Protect, Integrated Pest Management Innovat Ctr Hebei Prov, Key Lab IPM Crops Northern Reg North China,Minist, Baoding 071000, Peoples R China
关键词： Phenotype MicroArray; Bacillus subtilis; L-arabinose; sporulation; transcriptome
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.6； 五年影响因子：6.2 ）
ISSN： 1661-6596
年卷期： 2023 年 24 卷 24 期
页码：
收录情况： SCI
摘要： A microbial fungicide developed from Bacillus subtilis NCD-2 has been registered for suppressing verticillium wilt in crops in China. Spores are the main ingredient of this fungicide and play a crucial role in suppressing plant disease. Therefore, increasing the number of spores of strain NCD-2 during fermentation is important for reducing the cost of the fungicide. In this study, five kinds of carbon sources were found to promote the metabolism of strain NCD-2 revealed via Biolog Phenotype MicroArray (PM) technology. L-arabinose showed the strongest ability to promote the growth and sporulation of strain NCD-2. L-arabinose increased the bacterial concentration and the sporulation efficiency of strain NCD-2 by 2.04 times and 1.99 times compared with D-glucose, respectively. Moreover, L-arabinose significantly decreased the autolysis of strain NCD-2. Genes associated with arabinose metabolism, sporulation, spore resistance to heat, and spore coat formation were significantly up-regulated, and genes associated with sporulation-delaying protein were significantly down-regulated under L-arabinose treatment. The deletion of msmX, which is involved in arabinose transport in the Bacillus genus, decreased growth and sporulation by 53.71% and 86.46% compared with wild-type strain NCD-2, respectively. Complementing the mutant strain by importing an intact msmX gene restored the strain's growth and sporulation.
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