文献类型： 外文期刊

作者： Zhang, Yanfang ¹ ; Guo, Shuchun ³ ; Shao, Ying ³ ; Zhao, Lingmin ¹ ; Xing, Linan ¹ ; Ge, Mingran ¹ ; Huo, Xiuwen ¹ ;

作者机构： 1.Inner Mongolia Agr Univ, Dept Hort, Hohhot 010011, Peoples R China

2.Inner Mongolia Agr Univ, Plant Protect Coll, Hohhot 010011, Peoples R China

3.Inner Mongolia Acad Agr & Anim Husb Sci, Hohhot 010031, Peoples R China

关键词： Yam; Tuber Development; Transcriptome; Differentially Expressed Genes; Starch Synthesis Metabolism

期刊名称：JOURNAL OF BIOBASED MATERIALS AND BIOENERGY （ 影响因子：0.708； 五年影响因子：0.739 ）

ISSN： 1556-6560

年卷期： 2021 年 15 卷 5 期

页码：

收录情况： SCI

摘要： Yam (Dioscorea opposita) is a kind of vegetables with important nutritional, medicinal and economic value. To reveal the relationship between starch synthesis and gene expression in yam tubers at gene transcription level, transcriptome profiling was conducted by RNA-Seq in Bikeqi yam (Dioscorea opposita Thunb.) tubers at five key developmental stages (105, 120, 135, 150, and 165 days after sowing, DAS). Based on transcriptome sequencing data, a total of 45,867 unigenes were obtained. The results showed that 135 days after sowing are the key period of starch accumulation. During yam tuber development, 1,941 candidate differentially expressed genes (DEGs) were successfully classified into three GO categories, respectively, and there were 292, 267 and 478 unigenes in cellular component, molecular function and biological process. There were 767, 90 and 73 DEGs enriched in metabolic, plant hormone signal transduction and Plant-pathogen interaction pathway by Kyoto Encyclopedia of Genes and Genmes (KEGG),individually. Especially 72 IP: 49.249.253.194 On: Fri, 12 Nov 2021 04:51:16 DEGs were enriched in starch and sucrose metabolism pathway.In this pathway, the metabolic Copyright: American Scientific Publishers process was mainly positive regulated by gens encoding sucrose synthase, glucose-1-phosphate Delivered by Ingenta adenylyltransferase, alpha-trehalase, and so on. There was negative regulated by genes encoding beta-glucosidase. 10 DEGs involved in starch synthesis were selected to prove the accuracy of the RNA-Seq data by qPCR, 85% (34/40) of the results were consistent. The results lay a theoretical foundation be used for further understanding the starch synthesis mechanism of yam tubers development and accelerating breeding progress.