文献类型： 外文期刊

作者： Zhao, Lingmin ¹ ; Zhang, Yanfang ¹ ; Shao, Ying ² ; Xing, Linan ¹ ; Ge, Mingran ¹ ; Huo, Xiuwen ¹ ;

作者机构： 1.Inner Mongolia Agr Univ, Fac Hort & Plant Protect Sci, Hort Dept, Hohhot 010019, Peoples R China

2.Inner Mongolia Acad Agr & Anim Husb Sci, Hohhot 010031, Peoples R China

关键词： yam; beta-amylase; DoBAM1; starch degradation; cold tolerance

期刊名称：GENES （ 影响因子：4.141； 五年影响因子：4.474 ）

ISSN：

年卷期： 2022 年 13 卷 12 期

页码：

收录情况： SCI

摘要： beta-amylase (BAM) plays an important role in plant development and response to abiotic stresses. In this study, 5 DoBAM members were identified in yam (Dioscorea opposita Thunb.). A novel beta-amylase gene BAM1, (named DoBAM1), was isolated from yam varieties Bikeqi and Dahechangyu. The open reading frame (ORF) of DoBAM1 is 2806 bp and encodes 543 amino acids. Subcellular localization analysis indicates that DoBAM1 localizes to the cell membrane and cytoplasm. In the yam variety Dahechangyu, the starch content, beta-amylase activity, and expression of DoBAM1 were characterized and found to all be higher than in Bikeqi. DoBAM1 overexpression in tobacco is shown to promote the accumulation of soluble sugar and chlorophyll content and to increase the activities of peroxidase (POD), superoxide dismutase (SOD), catalase (CAT), and beta-amylase. Under cold treatment, we observed the induced upregulation of DoBAM1 and lower starch content and malondialdehyde (MDA) accumulation than in WT plants. In conclusion, these results demonstrate that DoBAM1 overexpression plays an advanced role in cold tolerance, at least in part by raising the levels of soluble sugars that are capable of acting as osmolytes or antioxidants.