Improving the Herbicide Resistance of Rice 4-Hydroxyphenylpyruvate Dioxygenase by DNA Shuffling Basis-Directed Evolution
文献类型： 外文期刊
作者： Chen, Le 1 ; Liu, Rui 1 ; Tan, Qing 1 ; Luo, Hongmei 1 ; Chen, Yuyu 1 ; Jin, Yaru 1 ; Zheng, Zhongbing 1 ; Zhang, Baolong 1 ; Guo, Dongshu 2 ;
作者机构： 1.Hainan Univ, Coll Trop Crops, Haikou 570228, Peoples R China
2.Jiangsu Acad Agr Sci, Inst Germplasm Resources & Biotechnol, Key Lab Jiangsu Prov Agrobiol, Nanjing 210014, Peoples R China
3.Zhongshan Biol Breeding Lab, Nanjing 210014, Peoples R China
关键词： 4-hydroxyphenylpyruvatedioxygenase (HPPD); directedevolution; DNA shuffling; enzyme properties; herbicide resistance
期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.1； 五年影响因子：6.3 ）
ISSN： 0021-8561
年卷期： 2023 年 71 卷 41 期
页码：
收录情况： SCI
摘要： 4-Hydroxyphenylpyruvate dioxygenase (HPPD) is an ideal target for herbicide resistance genetic engineering. In this study, a mutant MFRR-2 with mesotrione resistance was screened from an Oryza sativa HPPD and mutant- Zea mays HPPD DNA shuffling library. The enzyme properties showed that although the stability of the mutant decreased in vitro, the enzyme activity of MFRR-2 at the optimum temperature of 25 degree celsius was still equivalent to that of OsHPPD. Under 50 mu M mesotrione treatment, MFRR-2 enzyme activity remained at approximately 90%, while the enzyme activity of OsHPPD decreased by approximately 50%. Surprisingly, Fe2+ was found to have an inhibitory effect on the enzyme activity. Then, the transgenic rice of the MFRR-2 gene showed approximately 1.5 times mesotrione resistance compared to OsHPPD transgenic rice. In conclusion, this study has conducted a beneficial exploration on the use of DNA shuffling for HPPD-directed evolution, and the mutant has potential application value for herbicide resistance genetic engineering.
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