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BrPARP1, a Poly (ADP-Ribose) Polymerase Gene, Is Involved in Root Development in Brassica rapa under Drought Stress

文献类型： 外文期刊

作者： Cao, Gangqiang 1 ; Jiang, Wenjing 1 ; Shi, Gongyao 1 ; Tian, Zhaoran 1 ; Shang, Jingjing 1 ; Xie, Zhengqing 1 ; Chen, Weiwei 1 ; Tian, Baoming 1 ; Wei, Xiaochun 2 ; Wei, Fang 1 ; Gu, Huihui 1 ;

作者机构： 1.Zhengzhou Univ, Sch Agr Sci, Henan Int Joint Lab Crop Gene Resources & Improve, Zhengzhou 450001, Peoples R China

2.Grad T&R Base Zhengzhou Univ, Henan Acad Agr Sci, Inst Hort, Zhengzhou 450002, Peoples R China

关键词： poly (ADP-ribose) polymerase; drought; reactive oxygen species; root growth; whole-genome triplication

期刊名称：HORTICULTURAE （ 影响因子：2.923； 五年影响因子：3.582 ）

ISSN：

年卷期： 2022 年 8 卷 1 期

页码：

收录情况： SCI

摘要： PARP proteins are highly conserved homologs among the eukaryotic poly (ADP-ribose) polymerases. After activation, ADP-ribose polymers are synthesized on a series of ribozymes that use NAD+ as a substrate. PARPs participate in the regulation of various important biological processes, such as plant growth, development, and stress response. In this study, we characterized the homologue of PARP1 in B. rapa using RNA interference (RNAi) to reveal the underlying mechanism responding to drought stress. Bioinformatics and expression pattern analyses demonstrated that two copy numbers of PARP1 genes (BrPARP1.A03 and BrPARP1.A05) in B. rapa following a whole-genome triplication (WGT) event were retained compared with Arabidopsis, but only BrPARP1.A03 was predominantly transcribed in plant roots. Silencing of BrPARP1 could markedly promote root growth and development, probably via regulating cell division, and the transgenic Brassica lines showed more tolerance under drought treatment, accompanied with substantial alterations including accumulated proline contents, significantly reduced malondialdehyde, and increased antioxidative enzyme activity. In addition, the findings showed that the expression of stress-responsive genes, as well as reactive oxygen species (ROS)-scavenging related genes, was largely reinforced in the transgenic lines under drought stress. In general, these results indicated that BrPARP1 likely responds to drought stress by regulating root growth and the expression of stress-related genes to cope with adverse conditions in B. rapa.

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