Characterization of phosphofructokinase (PFK) from mud crab Scylla paramamosain and its role in mud crab dicistrovirus-1 proliferation
文献类型： 外文期刊
作者： Jie, Yu-Kun 1 ; Luo, Zhi-Ping 3 ; Xie, Jia-Wei 1 ; Cheng, Chang-Hong 1 ; Ma, Hong-Ling 1 ; Liu, Guang-Xin 1 ; Jiang, Jian-Jun 1 ; Deng, Yi-Qin 1 ; Feng, Juan 1 ; Guo, Zhi-Xun 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Key Lab South China Sea Fishery Resources Exploit, Minist Agr & Rural Affairs, South China Sea Fisheries Res Inst, Guangzhou 510300, Guangdong, Peoples R China
2.Shanghai Ocean Univ, Natl Demonstrat Ctr Expt Fisheries Sci Educ, Shanghai Engn Res Ctr Aquaculture, Shanghai 201306, Peoples R China
3.Zhuhai Modern Agr Dev Ctr, Zhuhai 519070, Guangdong, Peoples R China
关键词： Phosphofructokinase; Scylla paramamosain; Glycolysis; MCDV-1
期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.7； 五年影响因子：4.7 ）
ISSN： 1050-4648
年卷期： 2022 年 124 卷
页码：
收录情况： SCI
摘要： Phosphofructokinase (PFK), the key enzyme of glycolysis, can catalyze the irreversible transphosphorylation of fructose-6-phosphate forming fructose-1, 6-biphosphate. In the present study, a PFK gene from the mud crab Scylla paramamosain, named SpPFK, was cloned and characterized. The full length of SpPFK contained a 5 ' untranslated region (UTR) of 249 bp, an open reading frame of 2,859 bp, and a 3 ' UTR of 1,248 bp. The mRNA of SpPFK was highly expressed in the gill, followed by the hemocytes and muscle. The expression of SpPFK was significantly up-regulated after mud crab dicistrovirus-1 (MCDV-1) infection. Knocking down SpPFK in vivo by RNA interference significantly reduced the expression of lactate dehydrogenase after MCDV-1 infection. Furthermore, silencing of SpPFK in vivo increased the survival rate of mud crabs and decreased the MCDV-1 copies in the gill and hepatopancreas after MCDV-1 infection. All these results suggested that SpPFK could play an important role in the process of MCDV-1 proliferation in mud crab.
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