The First Telomere-to-Telomere Chromosome-Level Genome Assembly of Stagonospora tainanensis Causing Sugarcane Leaf Blight
文献类型： 外文期刊
作者： Xu, Fu 1 ; Li, Xiuxiu 2 ; Ren, Hui 1 ; Zeng, Rensen 1 ; Wang, Zhoutao 1 ; Hu, Hongli 2 ; Bao, Jiandong 3 ; Que, Youxiong 1 ;
作者机构： 1.Minist Agr & Rural Affairs, Key Lab Sugarcane Biol & Genet Breeding, Fuzhou 350002, Peoples R China
2.Fujian Agr & Forestry Univ, Coll Plant Protect, Fuzhou 350002, Peoples R China
3.Zhejiang Acad Agr Sci, Inst Plant Protect & Microbiol, State Key Lab Managing Biot & Chem Treats & Safet, Hangzhou 310021, Peoples R China
关键词： Stagonospora tainanensis; sugarcane leaf blight; pathogenicity; Nanopore sequencing; genome assembly
期刊名称：JOURNAL OF FUNGI （ 影响因子：5.724； 五年影响因子：6.413 ）
ISSN：
年卷期： 2022 年 8 卷 10 期
页码：
收录情况： SCI
摘要： The sexual morph Leptosphaeria taiwanensis Yen and Chi and its asexual morph Stagonospora tainanensis W. H. Hsieh is an important necrotrophic fungal phytopathogen, which causes sugarcane leaf blight, resulting in loss of cane tonnage and sucrose in susceptible sugarcane varieties. Decoding the genome and understanding of the basis of virulence is vitally important for devising effective disease control strategies. Here, we present a 38.25-Mb high-quality genome assembly of S. tainanensis strain StFZ01, denovo assembled with 10.19 Gb Nanopore sequencing long reads (similar to 267x) and 3.82 Gb Illumina short reads (similar to 100x). The genome assembly consists of 12 contigs with N50 of 2.86 Mb of which 5 belong to the telomere to telomere (T2T) chromosome. It contains 13.20% repeat sequences, 12,543 proteins, and 12,206 protein-coding genes with the BUSCO completeness 99.18% at fungi (n = 758) and 99.87% at ascomycota (n = 1706), indicating the high accuracy and completeness of our gene annotations. The virulence analysis in silico revealed the presence of 2379 PHIs, 599 CAZys, 248 membrane transport proteins, 191 cytochrome P450 enzymes, 609 putative secreted proteins, and 333 effectors in the StFZ01 genome. The genomic resources presented here will not only be helpful for development of specific molecular marker and diagnosis technique, population genetics, molecular taxonomy, and disease managements, it can also provide a significant precise genomic reference for investigating the ascomycetous genome, the necrotrophic lifestyle, and pathogenicity in the future.
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