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HOG1 Mitogen-Activated Protein Kinase Pathway-Related Autophagy Induced by H2O2 in Lentinula edodes Mycelia

文献类型： 外文期刊

作者： Yan, Dong 1 ; Fan, Yangyang 1 ; Song, Shuang 1 ; Guo, Yuan 1 ; Liu, Yu 1 ; Xu, Xiaoling 2 ; Liu, Fang 2 ; Gao, Qi 1 ; Wang, Shouxian 1 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Inst Plant Protect, Beijing Engn Res Ctr Edible Mushroom, 9 Shuguang Garden Zhonglu, Beijing 100097, Peoples R China

2.Baise Univ, Coll Agr & Food Engn, 21 Zhongshan Second St, Baise 533000, Peoples R China

关键词： Lentinula edodes; H2O2; autophagy; LeATG8; LeHOG1

期刊名称：JOURNAL OF FUNGI （ 影响因子：4.7； 五年影响因子：5.2 ）

ISSN：

年卷期： 2023 年 9 卷 4 期

页码：

收录情况： SCI

摘要： Mycelial ageing is associated with ROS and autophagy in Lentinula edodes. However, the underlying cellular and molecular mechanisms between ROS and autophagy remain obscure. This study induced autophagy in L. edodes mycelia through exogenous H2O2 treatment. Results showed that 100 mu M H2O2 treatment for 24 h significantly inhibited mycelial growth. H2O2 caused the depolarisation of MMP and accumulation of TUNEL-positive nuclei, which was similar to the ageing phenotype of L. edodes mycelia. Transcriptome analysis showed that differentially expressed genes were enriched in the mitophagic, autophagic, and MAPK pathways. LeAtg8 and LeHog1 were selected as hub genes. RNA and protein levels of LeATG8 increased in the H2O2-treated mycelia. Using fluorescent labelling, we observed for the first time the classic ring structure of autophagosomes in a mushroom, while 3D imaging suggested that these autophagosomes surrounded the nuclei to degrade them at specific growth stages. Phospho-LeHOG1 protein can translocate from the cytoplasm to the nucleus to regulate mycelial cells, resisting ROS-induced oxidative stress. Furthermore, LeATG8 expression was suppressed when LeHOG1 phosphorylation was inhibited. These results suggest that the LeATG8-dependent autophagy in L. edodes mycelial is closely associated with the activity or even phosphorylation of LeHOG1.

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