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Molecular Characterization and Pathogenicity of a Novel Soybean-Infecting Monopartite Geminivirus in China

文献类型： 外文期刊

作者： Du, Min 1 ; Wang, Yongzhi 1 ; Chen, Cheng 1 ; Li, Xiaoyu 2 ; Feng, Runzi 1 ; Zhou, Xueping 1 ; Yang, Xiuling 1 ;

作者机构： 1.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China

2.Jilin Acad Agr Sci, Minist Agr, Key Lab Integrated Pest Management Crops Northeas, Changchun 130033, Peoples R China

3.Sichuan Acad Agr Sci, Minist Agr, Inst Plant Protect, Key Lab Integrated Pest Management Crops Southwes, Chengdu 610066, Peoples R China

4.Zhejiang Univ, Inst Biotechnol, State Key Lab Rice Biol, Hangzhou 310058, Peoples R China

关键词： geminivirus; soybean; recombination; ssDNA virus

期刊名称：VIRUSES-BASEL （ 影响因子：5.818； 五年影响因子：5.811 ）

ISSN：

年卷期： 2022 年 14 卷 2 期

页码：

收录情况： SCI

摘要： Soybean is a major legume crop that plays an important role in food production, industrial production, and animal husbandry. Here, we characterize a novel soybean-infecting monopartite geminivirus identified in China. Analysis of the contigs de novo assembled from sequenced small interfering RNAs, followed by PCR, cloning, and sequencing, the complete viral genome was determined to be 2782 nucleotides. The genome contains the conserved nonanucleotide sequence, TAATATTAC and other sequence features typical of the family Geminiviridae, and encodes two and four open reading frames in the virion-sense and the complementary-sense strands, respectively. Genome-wide pairwise identity analysis revealed that the novel virus shares less than 65.6% identity with previously characterized geminiviruses. Phylogenetic and recombination analysis indicated that this virus was placed in a unique taxon within the family Geminiviridae and potentially arose from recombination. An infectious clone of this virus was further constructed and its infectivity was tested in different species of plants. Successful infection and characteristic symptoms were observed in Glycine max, Nicotiana benthamiana, N. tabacum, N. glutinosa, and N. tabacum cv. Samsun plants. Taken together, this virus represents a member of an unclassified genus of the family Geminiviridae, for which the name soybean yellow leaf curl virus is proposed.

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