您好，欢迎访问黑龙江省农业科学院 机构知识库！
黑龙江省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

A Single Nucleotide Variation of CRS2 Affected the Establishment of Photosynthetic System in Rice

文献类型： 外文期刊

作者： Chen, Hongwei 1 ; Wang, Qi 1 ; Fan, Mingqian 1 ; Zhang, Xijuan 2 ; Feng, Pulin 1 ; Zhu, Lin 1 ; Wu, Jiayi 1 ; Cheng, Xiaoyi 1 ; Wang, Jiayu 1 ;

作者机构： 1.Shenyang Agr Univ, Rice Res Inst, Key Lab Rice Biol & Genet Breeding Northeast China, Minist Agr & Rural Areas, Shenyang 110866, Peoples R China

2.Heilongjiang Prov Engn Technol Res Ctr Crop Cold D, Cultivat & Tillage Inst, Heilongjiang Acad Agr Sci, Harbin 150086, Peoples R China

关键词： chloroplast RNA splicing; photosynthesis; chlorophyll fluorescence; map-based cloning; rice (Oryza sativa L.)

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.6； 五年影响因子：6.2 ）

ISSN：

年卷期： 2023 年 24 卷 6 期

页码：

收录情况： SCI

摘要： Chloroplasts are essential sites for plant photosynthesis, and the biogenesis of the photosynthetic complexes involves the interaction of nuclear genes and chloroplast genes. In this study, we identified a rice pale green leaf mutant, crs2. The crs2 mutant showed different degrees of low chlorophyll phenotypes at different growth stages, especially at the seedling stage. Fine mapping and DNA sequencing of crs2 revealed a single nucleotide substitution (G4120A) in the eighth exons of CRS2, causing a G-to-R mutation of the 229th amino acid of CRS2 (G229R). The results of complementation experiments confirmed that this single-base mutation in crs2 is responsible for the phenotype of the crs2 mutant. CRS2 encodes a chloroplast RNA splicing 2 protein localized in the chloroplast. Western blot results revealed an abnormality in the abundance of the photosynthesis-related protein in crs2. However, the mutation of CRS2 leads to the enhancement of antioxidant enzyme activity, which could reduce ROS levels. Meanwhile, with the release of Rubisco activity, the photosynthetic performance of crs2 was improved. In summary, the G229R mutation in CRS2 causes chloroplast protein abnormalities and affects photosystem performance in rice; the above findings facilitate the elucidation of the physiological mechanism of chloroplast proteins affecting photosynthesis.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：黑龙江省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台