Heterologous expression and characterization of a novel glycoside hydrolase family 55 beta-1,3-glucanase, AcGluA, from Archangium sp. strain AC19
文献类型： 外文期刊
作者： Wang, Yanxin 1 ; Li, Ding 2 ; Dong, Chaonan 1 ; Zhao, Yuqiang 1 ; Zhang, Lei 1 ; Yang, Fan 1 ; Ye, Xianfeng 1 ; Huang, Ya 1 ;
作者机构： 1.Nanjing Agr Univ, Coll Life Sci, Key Lab Agr Environm Microbiol, Minist Agr & Rural Affairs, Nanjing 210095, Peoples R China
2.Jiangsu Acad Agr Sci, Inst Vet Immunol & Engn, Nanjing 210014, Peoples R China
3.Jiangsu Prov & Chinese Acad Sci, Inst Bot, Nanjing Bot Garden Mem Sun Yat Sen, Nanjing 210014, Peoples R China
4.Nanjing Agr Univ, Key Lab Plant Immun, Nanjing 210095, Peoples R China
关键词： beta-1,3-glucanase; Glycoside hydrolase family 55; Oligosaccharides; Immunostimulatory; Rice; Magnaporthe oryzae
期刊名称：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：4.813； 五年影响因子：4.697 ）
ISSN： 0175-7598
年卷期： 2021 年 105 卷 18 期
页码：
收录情况： SCI
摘要： Some microbial-associated molecular patterns (MAMPs), like glucan oligosaccharides, can be recognized by pattern recognition receptors (PRRs) of plant to elicit further immunity response. In this study, a novel glycoside hydrolase family 55 beta-1,3-glucanase (AcGluA) from Archangium sp. strain AC19 was cloned and expressed in Escherichia coli. Among the reported beta-1, 3-glucanases from the glycoside hydrolase 55 family, the purified AcGluA exhibited the highest activity on laminarin at pH 6.0 and 60 degrees C with 112.3 U/mg. Activity of AcGluA was stable in the range of pH 4.0-9.0 and at temperatures below 60 degrees C. The K-m and V-max of AcGluA for laminarin were 3.5 mg/ml and 263.5 mu mol/(ml.min). AcGluA hydrolyzed laminarin into a series of oligosaccharides, suggesting it was an endo-beta-1,3-glucanase. The high dose of oligosaccharides (1600 mg/l) had conspicuous biocontrol efficacy on the defense of rice seedlings to Magnaporthe oryzae, which provided a new idea for the development of green biopesticide.
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