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Haploid induction in allotetraploid tobacco using DMPs mutation

文献类型： 外文期刊

作者： Zhang, Xiaolian 1 ; Zhang, Lili 2 ; Zhang, Jishun 1 ; Jia, Mengao 2 ; Cao, Linggai 2 ; Yu, Jing 1 ; Zhao, Degang 1 ;

作者机构： 1.Guizhou Univ, Key Lab Plant Resources Conservat & Germplasm Inn, Guizhou Key Lab Agrobioengn, Minist Educ,Inst AgroBioengn,Coll Life Sci, Guiyang 550025, Peoples R China

2.Guizhou Acad Tobacco Sci, Mol Genet Key Lab China Tobacco, Guiyang 550081, Peoples R China

3.Guizhou Acad Agr Sci, Guizhou Plant Conservat Technol Ctr, Guizhou Key Lab Agr Biotechnol, Guiyang 550006, Peoples R China

关键词： CRISPR; Cas9; dmp1dmp2dmp3; Haploid inducer; Polyploidy; Powdery mildew resistance

期刊名称：PLANTA （ 影响因子：4.54； 五年影响因子：4.689 ）

ISSN： 0032-0935

年卷期： 2022 年 255 卷 5 期

页码：

收录情况： SCI

摘要： Main conclusion dmp1dmp2dmp3 mutants created by CRISPR/Cas9 could trigger maternal haploids in the allotetraploid model plant Nicotiana tabacum L. Double haploid (DH) technology is becoming increasingly important because it can significantly accelerate the breeding process. Haploid induction plays a fundamental role in the production of DH lines. Haploid induction has been realized and applied in diploid plants using DMP genes. However, it has yet to be elucidated whether haploid induction could be established in polyploid plants. In the current study, three homologues of the DMP genes (NtDMP1, 2, and 3) were identified in the allotetraploid plant Nicotiana tabacum, and the encoded proteins localized in the endoplasmic reticulum. Loss-of-function mutations in all three genes triggered maternal haploids with an induction rate of 1.52-1.75%. Compared with wild-type tobacco, the created haploid inducer exhibited differences in pollen vigor and seed germination rate. Furthermore, to rapidly and easily screen haploids, a visible haploid identification system was established based on a powdery mildew resistance phenotype. Findings from this study lay the foundation for the potential application of haploid inducers in allotetraploid plants such as tobacco.

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