您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Gene expression profile analysis of testis and ovary of oriental river prawn, Macrobrachium nipponense, reveals candidate reproduction-related genes

文献类型： 外文期刊

作者： Qiao, H. 1 ; Xiong, Y. W. 1 ; Jiang, S. F. 1 ; Fu, H. T. 1 ; Sun, S. M. 1 ; Jin, S. B. 1 ; Gong, Y. S. 1 ; Zhang, W. Y. 2 ;

作者机构： 1.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Minist Agr, Key Lab Freshwater Fisheries & Germplasm Resource, Wuxi, Peoples R China

2.Chinese Acad Fishery Sci, Fres

关键词： Reproduction-related genes;Differentially expressed genes;Testis;Ovary;Macrobrachium nipponense

期刊名称：GENETICS AND MOLECULAR RESEARCH （ 影响因子：0.764； 五年影响因子：0.912 ）

ISSN： 1676-5680

年卷期： 2015 年 14 卷 1 期

页码：

收录情况： SCI

摘要： This study utilized high-throughput RNA sequencing technology to identify reproduction-and development-related genes of Macrobrachium nipponense by analyzing gene expression profiles of testis and ovary. More than 20 million 1 x 51-bp reads were obtained by Illumina sequencing, generating more than 7.7 and 11.7 million clean reads in the testis and ovary library, respectively. As a result, 10,018 unitags were supposed to be differentially expressed genes (DEGs) between ovary and testis. Compared to the ovary library, 4563 (45.5%) of these DEGs exhibited at least 6-fold upregulated expression, while 5455 (54.5%) DEGs exhibited at least 2-fold downregulated expression in the testis. The Gene Ontology (GO) enrichment analysis showed that 113 GO terms had potential molecular functions in reproduction. The Kyoto Encyclopedia of Genes and Genomes results revealed that the most important pathways may be relevant to reproduction and included 7 pathways. Forty-two genes were identified as reproduction-, development-, and sex-related genes based on GO classification and sequence comparison with other publications, including male reproductive-related LIM protein, spermatogenesis-associated protein, gametocyte-specific factor 1, VASA-like protein, vitellogenin, sex-determining protein fem-1, and other potential candidates. These results will advance research in the field of molecular genetics in M. nipponense and offer a valuable resource for further research related to reproduction in crustaceans.

  • 相关文献

[1]Identification and comparative profiling of ovarian and testicular microRNAs in the swimming crab Portunus trituberculatus. Liu, Ping. 2018

[2]Growth differentiation factor 9 (Gdf9) was localized in the female as well as male germ cells in a protogynous hermaphroditic teleost fish, ricefield eel Monopterus albus. Zhang, Lihong,He, Zhi,Wu, Yangsheng,Zhang, Weimin,Xie, Jun,Wang, Taixin.

[3]Identification and comparative analysis of piRNAs in ovary and testis of Nile tilapia (Oreochromis niloticus). Zhou, Yi,Zhong, Huan,Xiao, Jun,Luo, Yongju,Gan, Xi,Zhou, Yi,Yan, Jinpeng,Yan, Jinpeng,Yu, Fan.

[4]Constructing and random sequencing analysis of normalized cDNA library of testis tissue from oriental river prawn (Macrobrachium nipponense). Qiao, Hui,Fu, Hongtuo,Jin, Shubo,Wu, Yan,Jiang, Sufei,Gong, Yongsheng,Xiong, Yiwei.

[5]Molecular characterization and expression pattern of dmrt1 in the immature Chinese sturgeon Acipenser sinensis. Leng, X. Q.,Cao, H.,Leng, X. Q.,Du, H. J.,Cao, H.,Leng, X. Q.,Li, C. J..

[6]Primary structure of a novel gonadotropin-releasing hormone (GnRH) in the ovary of red swamp crayfish Procambarus clarkii. Guan, Zheng-Bing,Liao, Xiang-Ru,Shui, Yan,Xu, Zeng-Hong,Zhou, Xin,Zhou, Xin. 2014

[7]cDNA cloning and expression of Ubc9 in the developing embryo and ovary of oriental river prawn, Macrobrachium nipponense. Zhang, Fengying,Chen, Liqiao,Wu, Ping,Zhao, Weihong,Li, Erchao,Zhang, Fengying,Qin, Jianguang.

[8]Dietary n-3 LC-PUFAs affect lipoprotein lipase (LPL) and fatty acid synthase (FAS) activities and mRNA expression during vitellogenesis and ovarian fatty acid composition of female silver pomfret (Pampus argenteus) broodstock. Peng, S.,Shi, Z.,Gao, Q.,Zhang, C.,Wang, J..

[9]Molecular cloning and expression pattern of oriental river prawn (Macrobrachium nipponense) nitric oxide synthase. Rahman, N. M. A.,Fu, H. T.,Jin, S.,Bai, H. K.,Liang, G. X.,Gong, Y. S.,Wu, Y.,Fu, H. T.,Sun, S. M.,Qiao, H.,Jin, S.,Zhang, W. Y.,Xiong, Y. W.,Rahman, N. M. A.. 2016

[10]Molecular cloning and expression analysis of Fem1b from oriental river prawn Macrobrachium nipponense. Rahman, N. M. A.,Fu, H.,Bai, H.,Jiang, F. W.,Liang, G.,Jiang, F. F.,Wu, Y.,Fu, H.,Qiao, H.,Jin, S.,Zhang, W.,Sun, S.,Gong, Y.,Xiong, Y.,Rahman, N. M. A.. 2016

[11]Molecular cloning, characterization, and expression analysis of a Broad-Complex homolog during development in the oriental river prawn Macrobrachium nipponense. Jiang, S. F.,Sun, S. M.,Gong, Y. S.,Xiong, Y. W.,Qiao, H.,Zhang, W. Y.,Jin, S. B.,Fu, H. T.,Zhang, Y. P.. 2015

[12]EFFECT OF BISPHENOL A EXPOSURE ON THE EXPRESSIONS OF FIVE OVARY DEVELOPMENT RELATED GENES IN THE ORIENTAL RIVER PRAWN, MACROBRACHIUM NIPPONENSE (DECAPODA, PALAEMONIDAE). Li, Xiu,Zhang, Fengying,Jiang, Keji,Zhao, Ming,Song, Wei,Ma, Lingbo,Li, Xiu,Zhao, Weihong. 2018

[13]Identification of androgenic gland microRNA and their target genes to discover sex-related microRNA in the oriental river prawn, Macrobrachium nipponense. Jin, S. B.,Fu, H. T.,Jin, S. B.,Fu, H. T.,Jiang, S. F.,Xiong, Y. W.,Qiao, H.,Zhang, W. Y.,Gong, Y. S.,Wu, Y.. 2015

[14]Six chitinases from oriental river prawn Macrobrachium nipponense: cDNA characterization, classification and mRNA expression during post-embryonic development and moulting cycle. Zhang, Shiyong,Fu, Hongtuo,Jiang, Fengwei,Jin, Shubo,Jiang, Sufei,Xiong, Yiwei,Fu, Hongtuo,Sun, Shengming,Qiao, Hui,Zhang, Wenyi,Jin, Shubo,Gong, Yongsheng. 2014

[15]Calcium-calmodulin dependent protein kinase I from Macrobrachium nipponense: CDNA cloning and involvement in molting. Shen, Huaishun,Hu, Yacheng,Zhang, Yuanqin,Zhou, Xin,Xu, Zenghong,Shen, Huaishun,Hu, Yacheng,Zhang, Yuanqin.

[16]Molecular cloning of two tropomyosin family genes and expression analysis during development in oriental river prawn, Macrobrachium nipponense. Jin, Shubo,Fu, Hongtuo,Jin, Shubo,Jiang, Sufei,Xiong, Yiwei,Qiao, Hui,Sun, Shengming,Zhang, Wenyi,Li, Fajun,Gong, Yongsheng,Fu, Hongtuo.

[17]Ecdysone receptor gene from the freshwater prawn Macrobrachium nipponense: Identification of different splice variants and sexually dimorphic expression, fluctuation of expression in the molt cycle and effect of eyestalk ablation. Shen, Huaishun,Zhou, Xin,Bai, Aixu,Ren, Xiufang,Zhang, Yanping,Bai, Aixu,Ren, Xiufang.

[18]Identification and characterization of a Macrobrachium nipponense ferritin subunit regulated by iron ion and pathogen challenge. Sun, Shengming,Gu, Zhimin,Gu, Zhimin,Sun, Shengming,Fu, Hongtuo,Zhu, Jian,Ge, Xianping,Xuan, Fujun.

[19]Genetic diversity analysis of oriental river prawn, Macrobrachium nipponense, in Yellow River using microsatellite marker. Qiao, H.,Jiang, S. F.,Sun, M.,Gong, Y. S.,Xiong, Y. W.,Jin, S. B.,Fu, H. T.,Lv, D.. 2013

[20]Isolation and characterization of two novel C-type lectins from the oriental river prawn, Macrobrachium nipponense. Xiu, Yunji,Hou, Libo,Wang, Yinghui,Gu, Wei,Meng, Qingguo,Wang, Wen,Xiu, Yunji,Hou, Libo,Wang, Yinghui,Gu, Wei,Meng, Qingguo,Wang, Wen,Xiu, Yunji,Liu, Xiaoqian.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台