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A novel active transposon creates allelic variation through altered translation rate to influence protein abundance

文献类型： 外文期刊

作者： Chen, Guo 1 ; Wang, Ruilin 1 ; Jiang, Yizhe 1 ; Dong, Xiaoxiao 1 ; Xu, Jing 1 ; Xu, Qiang 1 ; Kan, Qiuxin 1 ; Luo, Zhixiang 1 ; Springer, Nathan M. 3 ; Li, Qing 1 ;

作者机构： 1.Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China

2.Xinjiang Acad Agr Sci, Inst Nucl & Biol Technol, Xinjiang Key Lab Crop Biotechnol Improvement & Ger, Urumqi 830091, Peoples R China

3.Univ Minnesota, Dept Plant & Microbial Biol, St Paul, MN 55108 USA

4.Hubei Hongshan Lab, Wuhan 430070, Peoples R China

期刊名称：NUCLEIC ACIDS RESEARCH （ 影响因子：14.9； 五年影响因子：16.4 ）

ISSN： 0305-1048

年卷期： 2023 年 51 卷 2 期

页码：

收录情况： SCI

摘要： Protein translation is tightly and precisely controlled by multiple mechanisms including upstream open reading frames (uORFs), but the origins of uORFs and their role in maize are largely unexplored. In this study, an active transposition event was identified during the propagation of maize inbred line B73. The transposon, which was named BTA for 'B73 active transposable element hAT', creates a novel dosage-dependent hypomorphic allele of the hexose transporter gene ZmSWEET4c through insertion within the coding sequence in the first exon, and results in reduced kernel size. The BTA insertion does not affect transcript abundance but reduces protein abundance of ZmSWEET4c, probably through the introduction of a uORF. Furthermore, the introduction of BTA sequence in the exon of other genes can regulate translation efficiency without affecting their mRNA levels. A transposon capture assay revealed 79 novel insertions for BTA and BTA-like elements. These insertion sites have typical euchromatin features, including low levels of DNA methylation and high levels of H3K27ac. A putative autonomous element that mobilizes BTA and BTA-like elements was identified. Together, our results suggest a transposon-based origin of uORFs and document a new role for transposable elements to influence protein abundance and phenotypic diversity by affecting the translation rate.

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