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Screening of universal DNA barcodes for identifying grass species of Gramineae

文献类型： 外文期刊

作者： Wang, Jianli 1 ; Yan, Zhenfei 2 ; Zhong, Peng 3 ; Shen, Zhongbao 1 ; Yang, Guofeng 2 ; Ma, Lichao 2 ;

作者机构： 1.Heilongjiang Acad Agr Sci, Pratacultural Sci Inst, Harbin, Peoples R China

2.Qingdao Agr Univ, Coll Grassland Sci, Qingdao, Peoples R China

3.Heilongjiang Acad Agr Sci, Branch Anim Husb & Vet, Qiqihar, Peoples R China

关键词： DNA barcoding; haplotype; rbcL; matK; trnL-F; ITS

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：6.627； 五年影响因子：7.255 ）

ISSN： 1664-462X

年卷期： 2022 年 13 卷

页码：

收录情况： SCI

摘要： There is currently international interest in applying DNA barcoding as a tool for plant species discrimination and identification. In this study, we evaluated the utility of four candidate plant DNA barcoding regions [rbcL, matK, trnL-F, and internal transcribed spacer (ITS)] in seven genera of Gramineae including Agropyron, Bromus, Elymus, Elytrigia, Festuca, Leymus, and Lolium. Fourteen accessions were analyzed, and matK and ITS showed the highest species, subspecies, and variety discriminatory power, each resolving 11 accessions. Species discrimination using rbcL and trnL-F was lower, resolving 7 and 8 accessions, respectively. Subspecies and variety discrimination using rbcL and trnL-F could not identify 4 accessions of Agropyron. A technical system can be established using the proposed DNA barcode to rapidly and reliably identify the seven genera of Gramineae. This study serves as a "useful reference" for identifying the genetic diversity of grass germplasm resources. DNA barcoding can be utilized to uncover the relatives of different species within the same family or between different families. It can also be used to determine the related groups of important herbage, turfgrass, and crops and provide crucial background information for discovering excellent genes and improving existing crop varieties.

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