Computationally-driven epitope identification of PEDV N-protein and its application in development of immunoassay for PEDV detection
文献类型： 外文期刊
作者： Pang, Junzeng 1 ; Tian, Xiangqin 2 ; Han, Xiao 1 ; Yuan, Jiakang 1 ; Li, Linyue 1 ; You, Yonghe 3 ; Zhou, Yanlin 3 ; Xing, Guangxu 4 ; Li, Renfeng 1 ; Wang, Ziliang 1 ;
作者机构： 1.Henan Inst Sci & Technol, Coll Anim Sci & Vet Med, Xinxiang 453003, Peoples R China
2.Xinxiang Med Univ, Henan Key Lab Med Tissue Regenerat, Xinxiang 453003, Peoples R China
3.Xinxiang Med Univ, Sanquan Coll, Xinxiang 453000, Peoples R China
4.Henan Acad Agr Sci, Key Lab Anim Immunol, Minist Agr, Henan Prov Key Lab Anim Immunol, Zhengzhou 450002, Peoples R China
关键词： Porcine epidemic diarrhea virus; Epitopes of N protein; Monoclonal antibody; Quantum dots; Immunochromatographic assay
期刊名称：JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS （ 影响因子：3.4； 五年影响因子：3.3 ）
ISSN： 0731-7085
年卷期： 2023 年 235 卷
页码：
收录情况： SCI
摘要： The nucleocapsid (N) protein is a suitable candidate for early diagnosis of porcine epidemic diarrhea virus (PEDV). Here, we identified the linear B-cell epitopes of the PEDV N-protein by integrating a computationalexperimental framework and constructed three-dimensional (3D) structure model of the N protein using the ColabFold program in Google Colaboratory. Furthermore, we prepared the monoclonal antibodies against the predicted epitopes and recombinant N protein, respectively, and selected pairing mAbs (named 9C4 and 3C5) to develop a double-antibody sandwich immunochromatographic test strip using CdSe/ZnS quantum dots (QDs)labelled 9C4 and 3C5 as capture and detection antibodies, respectively. This strip can specifically detect PEDV within 10 min with a detection limit of less than 6.25 x 103 TCID50/mL. In comparison with RT-PCR for testing 90 clinical samples, the relative sensitivity and specificity of the strip were found to be 98.0% and 100%, respectively, with a concordance rate of 98.9% and a kappa value of 0.978, indicating that QDs-ICTS is a reliable method for the application of PEDV detection in clinical samples.
- 相关文献
作者其他论文 更多>>
-
Palmitoylation enhances the stability of porcine epidemic diarrhea virus spike protein by antagonizing its degradation via chaperone-mediated autophagy to facilitate viral proliferation
作者：Qian, Qisheng;Zhang, Gaiping;Qian, Qisheng;Zhao, Shuang-shuang;Yang, Lei;Xing, Guangxu;Li, Rui;Qiao, Songlin;Zhang, Gaiping;Qian, Qisheng;Zhang, Gaiping;Chen, Yumei;Liang, Chao;Wang, Haili;Wang, Aiping
关键词：porcine epidemic diarrhea virus; spike protein; palmitoylation; protein stability; chaperone-mediated autophagy
-
Development of a Paper-Based Microfluidic Chip for Point-of-Care Detection of PEDV
作者：Li, Renfeng;Cao, Wenyan;Jiang, Jiaxin;Yuan, Jiakang;Li, Linyue;Wang, Ziliang;Tian, Xiangqin;You, Yonghe;Zhou, Yanlin;Wang, Fangyu
关键词：porcine epidemic diarrhea virus; loop-mediated isothermal amplification; paper-based microfluidic analytical device; point-of-care detection
-
PEDV Nsp14 induces mitophagy-mediated degradation of MAVS to antagonize host innate immunity and facilitate viral proliferation
作者：Yang, Lei;Zhang, Gaiping;Yang, Lei;Qian, Qisheng;Chen, Xin-xin;Xing, Guangxu;Qiao, Songlin;Li, Rui;Zhang, Gaiping;Yang, Lei;Zhang, Gaiping;Sun, Yan-gang;Zhang, Jia-qing;Xing, Bao-song
关键词：PEDV; Nsp14; mitophagy; IFN-beta; NDP52
-
Porcine reproductive and respiratory syndrome virus triggers Golgi apparatus fragmentation-mediated autophagy to facilitate viral self-replication
作者：Zhao, Shuang-shuang;Qian, Qisheng;Zhang, Gaiping;Zhao, Shuang-shuang;Qian, Qisheng;Chen, Xin-xin;Lu, Qingxia;Xing, Guangxu;Qiao, Songlin;Li, Rui;Zhang, Gaiping;Zhang, Gaiping
关键词：autophagy; GA fragmentation; GRASP65; Nsp2; PRRSV; RAB2; ULK1
-
Development of a visual detection method of porcine deltacoronavirus using loop-mediated isothermal amplification
作者：Li, Renfeng;Cao, Wenyan;Yuan, Jiakang;Li, Linyue;Wang, Ziliang;Zhou, Yanlin;Wang, Fangyu;Tian, Xiangqin
关键词：porcine; deltacoronavirus; loop-mediated isothermal amplification; visualization; detection
-
PRRSV non-structural protein 5 inhibits antiviral innate immunity by degrading multiple proteins of RLR signaling pathway through FAM134B-mediated ER-phagy
作者：Wang, Jing;Sun, Huiqin;Xu, Shixuan;Zhang, Gaiping;Wang, Jing;Sun, Huiqin;Li, Rui;Xu, Shixuan;Guo, Junqing;Xing, Guangxu;Jia, Bin;Qiao, Songlin;Chen, Xin-xin;Zhang, Gaiping;Zhang, Gaiping
关键词：PRRSV; ER-phagy; IFN-I; FAM134B; Nsp5
-
Precise location of three novel linear epitopes using the generated monoclonal antibodies against the Knob domain of FAdV-4 surface structural protein, fiber1
作者：Chai, Yongxiao;Zhang, Gaiping;Chai, Yongxiao;Jin, Qianyue;Zhu, Rongfang;Guo, Zhenhua;Lu, Qingxia;Chai, Shujun;Xing, Yunrui;Xing, Guangxu;Zhang, Gaiping;Han, Lu;Zhang, Gaiping
关键词：FAdV-4; fiber-1 knob protein; monoclonal antibodies; B cell epitopes; structural analysis
