文献类型： 外文期刊
作者： Li, Renfeng 1 ; Tian, Xiangqin 2 ; Cao, Wenyan 1 ; Jiang, Jiaxin 1 ; Yuan, Jiakang 1 ; Li, Linyue 1 ; You, Yonghe 3 ; Zhou, Yanlin 3 ; Wang, Ziliang 1 ; Wang, Fangyu 4 ;
作者机构： 1.Henan Inst Sci & Technol, Coll Anim Sci & Vet Med, Xinxiang 453003, Peoples R China
2.Xinxiang Med Univ, Henan Key Lab Med Tissue Regenerat, Xinxiang 453003, Peoples R China
3.Xinxiang Med Univ, Sanquan Coll, Xinxiang 453003, Peoples R China
4.Henan Acad Agr Sci, Inst Anim Hlth, Key Lab Anim Immunol, Zhengzhou 450002, Peoples R China
关键词： porcine epidemic diarrhea virus; loop-mediated isothermal amplification; paper-based microfluidic analytical device; point-of-care detection
期刊名称：VETERINARY SCIENCES （ 影响因子：2.3； 五年影响因子：2.4 ）
ISSN：
年卷期： 2025 年 12 卷 5 期
页码：
收录情况： SCI
摘要： PEDV poses a significant threat to the global swine industry, necessitating rapid and accurate diagnostic methods for effective disease management. In this study, we developed a foldable, easy-to-use paper-based microfluidic analytical device (mu PAD) for on-site detection of PEDV. The device seamlessly integrates paper-based nucleic acid enrichment, LAMP reaction, and visual lateral flow detection into a single platform. Key parameters, including nucleic acid extraction protocols, chromatographic channel configurations, colorimetric indicators, and reaction temperature and duration, were systematically optimized. The resulting LAMP-mu PAD assay detects PEDV within 30 min at 60 degrees C, achieving a limit of detection of 4.82 x 102 copies/mu L with no cross-reactivity against other viruses. When evaluated against RT-PCR using clinical specimens, the assay demonstrated a specificity of 100%, a sensitivity of 95.3%, and an overall concordance of 98.5%. This paper-based sensor offers a promising alternative for the rapid, on-site detection of PEDV and other highly transmissible pathogens.
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