您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Purification and Characterization of a Novel Fibrinolytic Enzyme from Marine Bacterium Bacillus sp. S-3685 Isolated from the South China Sea

文献类型： 外文期刊

作者： Ma, Zibin 1 ; Elango, Jeevithan 2 ; Hao, Jianhua 5 ; Wu, Wenhui 2 ;

作者机构： 1.Taizhou Vocat Coll Sci & Technol, Sch Agr & Bioengn, Taizhou 318020, Peoples R China

2.Shanghai Ocean Univ, Coll Food Sci & Technol, Dept Marine Biopharmacol, Shanghai 201306, Peoples R China

3.Saveetha Univ, Ctr Mol Med & Diag COMManD, Saveetha Inst Med & Tech Sci, Saveetha Dent Coll & Hosp,Dept Biochem, Chennai 600077, India

4.UCAM Univ Catolica San Antonio Murcia, Fac Hlth Sci, Dept Biomat Engn, Murcia 30107, Guadalupe, Spain

5.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Dev Polar Fishery, Minist Agr & Rural Affairs, Qingdao 266071, Peoples R China

关键词： fibrinolytic enzyme; Bacillus sp.; purification; optimum activity; thrombolysis

期刊名称：MARINE DRUGS （ 影响因子：4.9； 五年影响因子：5.2 ）

ISSN：

年卷期： 2024 年 22 卷 6 期

页码：

收录情况： SCI

摘要： A novel fibrinolytic enzyme, BSFE1, was isolated from the marine bacterium Bacillus sp. S-3685 (GenBank No.: KJ023685) found in the South China Sea. This enzyme, with a molecular weight of approximately 42 kDa and a specific activity of 736.4 U/mg, exhibited its highest activity at 37 degrees C in a phosphate buffer at pH 8.0. The fibrinolytic enzyme remained stable over a pH range of 7.5 to 10.0 and retained about 76% of its activity after being incubated at 37 degrees C for 2 h. The K-m and V-max values of the enzyme at 37 degrees C were determined to be 2.1 mu M and 49.0 mu mol min(-1) mg(-1), respectively. The fibrinolytic activity of BSFE1 was enhanced by Na+, Ba2+, K+, Co2+, Mn2+, Al3+, and Cu2+, while it was inhibited by Fe3+, Ca2+, Mg2+, Zn2+, and Fe2+. These findings indicate that the fibrinolytic enzyme isolated in this study exhibits a strong affinity for fibrin. Moreover, the enzyme we have purified demonstrates thrombolytic enzymatic activity. These characteristics make BSFE1 a promising candidate for thrombolytic therapy. In conclusion, the results obtained from this study suggest that our work holds potential in the development of agents for thrombolytic treatment.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台