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QTL Mapping-Based Identification of Visceral White-Nodules Disease Resistance Genes in Larimichthys polyactis

文献类型： 外文期刊

作者： Li, Qian 1 ; Zhu, Jiajie 2 ; Liu, Sifang 2 ; Liu, Haowen 2 ; Zhang, Tianle 2 ; Ye, Ting 2 ; Lou, Bao 2 ; Liu, Feng 2 ;

作者机构： 1.Zhejiang Ocean Univ, Natl Engn Res Ctr Marine Aquaculture, Zhoushan 316022, Peoples R China

2.Zhejiang Acad Agr Sci, Inst Hydrobiol, Zhejiang Key Lab Coastal Biol Germplasm Resources, Hangzhou 310021, Peoples R China

3.Ningbo Univ, Sch Marine Sci, Key Lab Appl Marine Biotechnol Minist Educ, Ningbo 315211, Peoples R China

4.China Jiliang Univ, Coll Life Sci, Hangzhou 310018, Peoples R China

关键词： Pseudomonas plecoglossicida; Larimichthys polyactis; genetic linkage map; QTL mapping; disease resistance

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：4.9； 五年影响因子：5.7 ）

ISSN： 1661-6596

年卷期： 2024 年 25 卷 20 期

页码：

收录情况： SCI

摘要： Disease outbreaks in aquaculture have recently intensified. In particular, visceral white-nodules disease, caused by Pseudomonas plecoglossicida, has severely hindered the small yellow croaker (Larimichthys polyactis) aquaculture industry. However, research on this disease is limited. To address this gap, the present study employed a 100K SNP chip to genotype individuals from an F1 full-sib family, identify single nucleotide polymorphisms (SNPs), and construct a genetic linkage map for this species. A high-density genetic linkage map spanning a total length of 1395.72 cM with an average interval of 0.08 cM distributed across 24 linkage groups was obtained. Employing post-infection survival time as an indicator of disease resistance, 13 disease resistance-related quantitative trait loci (QTLs) were detected, and these regions included 169 genes. Functional enrichment analyses pinpointed 11 candidate disease resistance-related genes. RT-qPCR analysis revealed that the genes of chmp1a and arg1 are significantly differentially expressed in response to P. plecoglossicida infection in spleen and liver tissues, indicating their pivotal functions in disease resistance. In summary, in addition to successfully constructing a high-density genetic linkage map, this study reports the first QTL mapping for visceral white-nodules disease resistance. These results provide insight into the intricate molecular mechanisms underlying disease resistance in the small yellow croaker.

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