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Alternative Splicing Isoforms of Porcine CREB Are Differentially Involved in Transcriptional Transactivation

文献类型： 外文期刊

作者： Zhang, Dongjie 1 ; Zhang, Qian 2 ; Wang, Liang 1 ; Li, Jiaxin 2 ; Hao, Wanjun 2 ; Sun, Yuanlu 2 ; Liu, Di 1 ; Yang, Xiuqin 2 ;

作者机构： 1.Heilongjiang Acad Agr Sci, Inst Anim Husb, Harbin 150086, Peoples R China

2.Northeast Agr Univ, Coll Anim Sci & Technol, Harbin 150030, Peoples R China

关键词： pig; CREB; alternative splicing variants; function; RNA-seq

期刊名称：GENES （ 2021影响因子：4.141； 五年影响因子：4.474 ）

ISSN：

年卷期： 2022 年 13 卷 8 期

页码：

收录情况： SCI

摘要： The cAMP response element-binding protein (CREB), a basic leucine zipper transcription factor, is involved in the activation of numerous genes in a variety of cell types. The CREB gene is rich in alternative splicing (AS) events. However, studies on the AS of CREB genes in pigs are limited, and few reports have compared the roles of isoforms in activating gene expression. Here, five AS transcripts, V1-5, were characterized by RT-PCR and two, V3 and V5, were new identifications. Both V1 and V2 have all the functional domains of the CREB protein, with similar tissue expression profiles and mRNA stability, suggesting that they have similar roles. The transcriptional transactivation activities of four isoforms encoding complete polypeptides were analyzed on the expression of the B-cell CLL/lymphoma 2-like protein 2 and the poly (A)-binding protein, nuclear 1 genes with a dual-luciferase reporter system, and differential activities were observed. Both V1 and V2 have promoting effects, but their roles are gene-specific. V3 has no effect on the promoter of the two genes, while V4 functions as a repressor. The mechanisms underlying the differential roles of V1 and V2 were analyzed with RNA-seq, and the genes specifically regulated by V1 and V2 were identified. These results will contribute to further revealing the role of CREB and to analyzing the significance of AS in genes.

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