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Identification and Transcriptome Analysis of a Novel Allelic Mutant of NAL1 in Rice

文献类型： 外文期刊

作者： Wang, Yang 1 ; Xu, Wanxin 1 ; Liu, Yan 1 ; Yang, Jie 1 ; Guo, Xin 1 ; Zhang, Jiaruo 1 ; Pu, Jisong 1 ; Chen, Nenggang 3 ; Zhang, Wenfeng 1 ;

作者机构： 1.Xichang Univ, Coll Agr Sci, Panxi Crops Res & Utilizat Key Lab Sichuan Prov, Liangshan 615013, Peoples R China

2.Sichuan Agr Univ, State Key Lab Crop Gene Explorat & Utilizat Southw, Chengdu 611130, Peoples R China

3.Guizhou Acad Agr Sci, Inst Crop Germplasm Resources, Guiyang 550006, Peoples R China

关键词： rice; narrow leaf allelic mutant; gene cloning; NAL1; RNA-Seq

期刊名称：GENES （ 影响因子：3.5； 五年影响因子：3.9 ）

ISSN：

年卷期： 2024 年 15 卷 3 期

页码：

收录情况： SCI

摘要： Leaf morphology is a crucial aspect of plant architecture, yet the molecular mechanisms underlying leaf development remain incompletely understood. In this study, a narrow leaf mutant, m625, was identified in rice (Oryza sativa L.), exhibiting pleiotropic developmental defects. Pigment measurement revealed reduced levels of photochromic pigments in m625. Cytological analysis demonstrated that the m625 gene affected vascular patterns and cell division. Specifically, the narrowing of the leaf was attributed to a decrease in small vein number, shorter vein spacing, and an abnormal V-shaped arrangement of bulliform cells, while the thickening was caused by longer leaf veins, thicker mesophyll cells, and an increased number of parenchyma cell layers. The dwarf stature and thickened internode were primarily due to shortened internodes and an increase in cell layers, respectively. Positional cloning and complementation assays indicated that the m625 gene is a novel allele of NAL1. In the m625 mutant, a nucleotide deletion at position 1103 in the coding sequence of NAL1 led to premature termination of protein translation. Further RNA-Seq and qRT-PCR analyses revealed that the m625 gene significantly impacted regulatory pathways related to IAA and ABA signal transduction, photosynthesis, and lignin biosynthesis. Moreover, the m625 mutant displayed thinner sclerenchyma and cell walls in both the leaf and stem, particularly showing reduced lignified cell walls in the midrib of the leaf. In conclusion, our study suggests that NAL1, in addition to its known roles in IAA transport and leaf photosynthesis, may also participate in ABA signal transduction, as well as regulate secondary cell wall formation and sclerenchyma thickness through lignification.

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