Regulation of bta-miRNA29d-3p on Lipid Accumulation via GPAM in Bovine Mammary Epithelial Cells
文献类型： 外文期刊
作者： Zhao, Xin 1 ; Li, Jun 3 ; Zhao, Shuying 4 ; Chen, Lili 1 ; Zhang, Man 1 ; Ma, Yi 1 ; Yao, Dawei 1 ;
作者机构： 1.Tianjin Acad Agr Sci, Inst Anim Sci & Vet, Tianjin Engn Res Ctr Anim Hlth Farming, Tianjin Key Lab Anim Mol Breeding & Biotechnol, Tianjin 300381, Peoples R China
2.Nankai Univ, Coll Life Sci, Tianjin 300071, Peoples R China
3.Henan Univ Anim Husb & Econ, Coll Anim Sci & Technol, Zhengzhou 450046, Peoples R China
4.Tianjin Agr Univ, Coll Anim Sci & Anim Med, Tianjin 300384, Peoples R China
关键词： miR-29; lipid metabolism; GPAM; milk fat; cow mammary cells
期刊名称：AGRICULTURE-BASEL （ 影响因子：3.6； 五年影响因子：3.6 ）
ISSN：
年卷期： 2023 年 13 卷 2 期
页码：
收录情况： SCI
摘要： MicroRNAs (miRNAs) are small RNA molecules consisting of approximately 22 nucleotides that are engaged in the regulation of various bio-processes. There is growing evidence that miR-29 is a key regulator of hepatic lipid metabolism. Mimics and inhibitors of bta-miRNA29d-3p were transiently transfected in bovine mammary epithelial cells (BMECs) to reveal the regulation of bta-miRNA29d-3p on lipid accumulation in BMECs. Results showed that overexpression of bta-miRNA29d-3p significantly inhibited the expression of genes related to triglyceride (TAG) synthesis, namely DGAT1 and mitochondrial glycerol-3-phosphate acyltransferase (GPAM, p < 0.01) and down-regulated TAG levels in cells (p < 0.05). The expression of fatty acid synthesis and desaturation-related genes FASN, SCD1, and ACACA, and transcription factor SREBF1 also decreased. Interference of bta-miRNA29d-3p significantly increased the expression of GPAM, DGAT1, FASN, SCD1, ACACA, and SREBF1 (p < 0.01), and significantly upregulated the concentration of TAG in cells. Furthermore, a luciferase reporter assay confirmed that GPAM is a direct target of bta-miRNA29d-3p. In summary, bta-miRNA29d-3p modulates fatty acid metabolism and TAG synthesis by regulating genes related to lipid metabolism in BMECs and targeting GPAM. Thus, bta-miRNA29d-3p plays an important role in controlling mammary lipid synthesis in cows.
- 相关文献
作者其他论文 更多>>
-
Identification of diagnostic biomarkers of and immune cell infiltration analysis in bovine respiratory disease
作者：Sheng, Hui;Shi, Xiaodi;Zhang, Long;Yao, Dawei;Zhang, Peipei;Li, Yupeng;Zhang, Jinlong;Guo, Xiaofei;Zhang, Xiaosheng;Zhang, Junxing;Zhang, Long
关键词：bovine respiratory disease; lifetime productivity; potential biomarkers; immune infiltration; CIBERSORT
-
Full-length transcriptome sequencing of the longissimus dorsi muscle of yak and cattle-yak using nanopore technology
作者：Cao, Mengli;Ding, Ziqiang;Guo, Shaoke;Kang, Yandong;Hu, Liyan;Zhang, Ben;Pei, Jie;Guo, Xian;Cao, Mengli;Ding, Ziqiang;Guo, Shaoke;Kang, Yandong;Hu, Liyan;Zhang, Ben;Pei, Jie;Guo, Xian;Wang, Xingdong;Ma, Yi
关键词：Yak; Cattle-yak; Longissimus dorsi; Alternative splicing; Full-length transcriptom
-
Genome-Wide Association Analysis of Reproductive Traits in Chinese Holstein Cattle
作者：Liu, Jiashuang;Ma, Yi;Liu, Jiashuang;Ding, Xiangbin;Xu, Lingyang
关键词：genome-wide association study; reproductive traits; Chinese Holstein cattle
-
The Combined Analysis of GC-IMS and GC-MS Reveals the Differences in Volatile Flavor Compounds between Yak and Cattle-Yak Meat
作者：Zhang, Ben;Cao, Mengli;Wang, Xingdong;Guo, Shaoke;Ding, Ziqiang;Kang, Yandong;Hu, Liyan;Xiong, Lin;Pei, Jie;Guo, Xian;Zhang, Ben;Cao, Mengli;Wang, Xingdong;Guo, Shaoke;Ding, Ziqiang;Kang, Yandong;Hu, Liyan;Xiong, Lin;Pei, Jie;Guo, Xian;Ma, Yi
关键词：yak; cattle-yak; GC-IMS; GC-MS; VOCs; metabolites
-
MiR-206 Suppresses Triacylglycerol Accumulation via Fatty Acid Elongase 6 in Dairy Cow Mammary Epithelial Cells
作者：Zhao, Xin;Liu, Yu;Li, Yupeng;Zhang, Yuxin;Yang, Chunlei;Yao, Dawei
关键词：miR-206; ELOVL6; mammary epithelial cells; lipid metabolism
-
A Comparison of Growth Performance, Blood Parameters, Rumen Fermentation, and Bacterial Community of Tibetan Sheep When Fattened by Pasture Grazing versus Stall Feeding
作者：Ji, Huiying;Chen, Lili;Ma, Yi;Ji, Huiying;Chen, Hualong;Zhou, Jianwei;Degen, Abraham Allan;Yuan, Zhengrong
关键词：Tibetan sheep; fattening strategies; blood parameters; rumen bacterial community; rumen fermentation
-
Genome-wide association analysis of body conformation traits in Chinese Holstein Cattle
作者：Li, Shuangshuang;Chen, Lili;Ma, Yi;Li, Shuangshuang;Li, Shuangshuang;Ge, Fei;Liu, Yuxin;Chen, Yan
关键词：Chinese Holstein cattle; Body conformation traits; Single-trait GWAS; Multi-trait GWAS
