Regulation of bta-miRNA29d-3p on Lipid Accumulation via GPAM in Bovine Mammary Epithelial Cells
文献类型： 外文期刊
作者： Zhao, Xin 1 ; Li, Jun 3 ; Zhao, Shuying 4 ; Chen, Lili 1 ; Zhang, Man 1 ; Ma, Yi 1 ; Yao, Dawei 1 ;
作者机构： 1.Tianjin Acad Agr Sci, Inst Anim Sci & Vet, Tianjin Engn Res Ctr Anim Hlth Farming, Tianjin Key Lab Anim Mol Breeding & Biotechnol, Tianjin 300381, Peoples R China
2.Nankai Univ, Coll Life Sci, Tianjin 300071, Peoples R China
3.Henan Univ Anim Husb & Econ, Coll Anim Sci & Technol, Zhengzhou 450046, Peoples R China
4.Tianjin Agr Univ, Coll Anim Sci & Anim Med, Tianjin 300384, Peoples R China
关键词： miR-29; lipid metabolism; GPAM; milk fat; cow mammary cells
期刊名称：AGRICULTURE-BASEL （ 影响因子：3.6； 五年影响因子：3.6 ）
ISSN：
年卷期： 2023 年 13 卷 2 期
页码：
收录情况： SCI
摘要： MicroRNAs (miRNAs) are small RNA molecules consisting of approximately 22 nucleotides that are engaged in the regulation of various bio-processes. There is growing evidence that miR-29 is a key regulator of hepatic lipid metabolism. Mimics and inhibitors of bta-miRNA29d-3p were transiently transfected in bovine mammary epithelial cells (BMECs) to reveal the regulation of bta-miRNA29d-3p on lipid accumulation in BMECs. Results showed that overexpression of bta-miRNA29d-3p significantly inhibited the expression of genes related to triglyceride (TAG) synthesis, namely DGAT1 and mitochondrial glycerol-3-phosphate acyltransferase (GPAM, p < 0.01) and down-regulated TAG levels in cells (p < 0.05). The expression of fatty acid synthesis and desaturation-related genes FASN, SCD1, and ACACA, and transcription factor SREBF1 also decreased. Interference of bta-miRNA29d-3p significantly increased the expression of GPAM, DGAT1, FASN, SCD1, ACACA, and SREBF1 (p < 0.01), and significantly upregulated the concentration of TAG in cells. Furthermore, a luciferase reporter assay confirmed that GPAM is a direct target of bta-miRNA29d-3p. In summary, bta-miRNA29d-3p modulates fatty acid metabolism and TAG synthesis by regulating genes related to lipid metabolism in BMECs and targeting GPAM. Thus, bta-miRNA29d-3p plays an important role in controlling mammary lipid synthesis in cows.
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